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What is the purpose of each reagent in
- Primary stain
- Mordant
- Decolorizing agent
- Counterstain
Step by step
Solved in 2 steps
- Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methodsDescribe how the Gram stain is performed and identify the staining characteristics of Gram(+) and Gram(-)organisms. Be sure to include the lenght of time for each step,if water is used to rinse, and identify which step is most critical to doing a quality stain.Name the reagents used in Gram-staining and their roles in the staining procedure
- list the stains and decolorizing agent used in acid fast staining method.What is the principle behind the production of two different stain using Gram’s staining methodExplain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a day
- Why is it essential that the primary stain and the counterstain be contrasting colors in gram-staining?What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide? Paragraph BIUDraw what you would expect to see for acid fast stain and endospore stain procedure if you did not stain with counterstain. Color pencils need to be used.
- With what kind of gram stain do you use heat fixation? What is the purpose of heat fixation? What solution do you use to wash a positive stain? A negative stain?Which type of stain would be the best choice for negative staining of most bacterial cells: a basic stain or an acidic stain?List the reagents of the Gram stain technique in order and their general role in the staining process.