Q: How was the process of bacterial transformation discovered?
A: The reception of bare DNA fragments from the external environment and the manifestation of that…
Q: Explain how are the physiological characteristics of bacteria identified using biochemical test?
A: The physiological properties that are used for the identification of bacteria includes the nutrient…
Q: Why do we need to look up values in the charts for each antibiotic?
A: Antibiotics have definitely transformed the lives of countless individuals by rescuing them from…
Q: What conditions may have promoted bacterial growth?
A: There are some conditions that may have promoted bacterial growth. Here we will discuss the factors…
Q: Who discovered the plasmids?
A: Plasmid is an extrachromosomal small DNA molecule present in a cell. Plasmid exists separately from…
Q: What is the difference between complete medium and minimal medium? How are complete media and…
A: Nutritional requirements of bacteria and the culture media are defined by microbiologists. The…
Q: What are Koch's postulates? Discuss the importance of these postulates in the identification of…
A: As per Germ theory of Disease , pathogen ( disease causing agents) are the chief factors behind a…
Q: Why is pure culture and culture collection important in identifying novel species of bacteria?
A: A pure culture is a population of cells or multicellular organisms growing in the absence of other…
Q: What is the purpose of the bacterial capsule? Answer in not more than 3 sentences.
A: Prokaryotic calls contain addition protective layer outside of the bacterial cell wall. It is…
Q: Why each bacterium must be tested separately?
A: Antibiotics are chemical substances that kill or inhibit or prevent the growth of microbes. They are…
Q: What are challenges or difficulties we may encounter for identifying a bacillus cereus microbe ?
A: Bacillus cereus is a gram-positive, facultatively anaerobe, and the rod-shaped bacterium present in…
Q: What difficulties does one encounter when trying to differentiate bacteria on the basis of…
A: Bacteria are differentiated based on many different characteristics such as cell shape, nature of…
Q: If the seven-digit code that is tabulated cannot be found in the Profile Determination Table, what…
A: The bacterial identification is important to ensure that the unknown bacterial strain is correctly…
Q: Based on the results of the Kirby-Bauer test shown below, what can we say about this strain of…
A: In Kirby-Bauer testing, bacteria are put on a plate of solid growth medium and wafers of antibiotics…
Q: What tools are used to transfer bacteria
A: Ans - An inoculation loop is used to transfer bacteria for microbiological culture. Petri dish.…
Q: How was Escherichia coli modified to produce indigo?
A: Indigo is a dye used mainly for coloring textiles and is derived from plant materials. With the…
Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: What are the advantages and disadvantages of multitest systems for bacterial identification?
A: A multitest system is the combination of multiple biochemical tests useful in identification for the…
Q: What bacteria is gram negative?
A: Bacteria are member of a large group of unicellular microorganism. It has cell walls but lack…
Q: Can the presence and absence of capsules in bacteria be used to identify unknown bacteria? YES OR…
A: Glycocalyx is present external to the cell wall as an outermost covering of bacteria. It protects…
Q: What is the procedure of beta method?
A: Beta-lactamase test is used for the detection of enzyme beta-lactamase. This enzyme provides the…
Q: When using a probe to detect an organism, why is it necessary to have some idea as to the organism’s…
A: DNA is the genetic material in all living organisms. Each organism has a unique DNA sequence. Using…
Q: How many types of bacteria are there?
A: Bacteria is the most important component of the cellular life on Earth and are found everywhere from…
Q: What is the purpose of making a serial dilution?
A: Given: In microbiology, serial dilutions (log dilutions) are used to reduce the bacterial…
Q: How can a suspect bacterium be fingerprinted?
A: DNA fingerprinting is a technique for detecting a high number of minisatellites in the genome at the…
Q: What are other methods of measuring the sensitivity of organisms to antibiotic?
A: An antibiotic is a substance that kills or inhibits the growth of bacteria and other microorganisms,…
Q: When using the blue/ white colony screening method, what do blue colonies represent ?
A: Blue- white screening is used for the identification of recombinant bacteria.
Q: What is the principle of the gel electrophoresis separation technique?
A: Biological techniques are procedures or strategies for studying biological things. Biological…
Q: What is a quantitative isolation in bacterial cultivation and how it is done? Explain.
A: To cultivate and count the bacteriophage, plaque formation in solid agar media is used. Plaques are…
Q: How was Escherichia coli modified to produce a photograph?
A: Bacteria are a group of prokaryotic microscopic single celled organisms. They live in diverse…
Q: What biochemical tests did we use to distinguish staphylococci from streptococci?
A: Staphylococcus and streptococcus both occur in cocci shape and both are gram positive organisms.…
Q: If the bacterium was lysogenized by a bacteriophage that has a virulence gene, determine the kind of…
A: The phenotypic approaches involve identifying bacteria by taking into account their behaviour and…
Q: What is the correct genus and species of your unknown Gram (+) bacterium?
A: Mannitol Salt Agar (MSA) It is used to determine halophilic and can fermenting mannitol ability of…
Q: What is the procedure for methylene blue stain on e.coli?
A: Methylene Blue is a cationic stain, which binds to negatively charged parts of the cells, such as…
Q: Bacterial mutants can be classified on the basis of the method, Which is those methods?
A: Microorganisms undergo mutations under environmental stress that causes sudden heritable changes in…
Q: Which Part Of The Bacteria Actually Gets Stained?
A: The primary goal of staining is to show cytological details that would otherwise go undetected;…
Q: If you had a liquid culture containing three different species of bacteria, how could the use of a…
A: A microbiological culture, also known as a microbial culture, is a technique for multiplying…
Q: What are the advantages and limitations of studying bacteria by means of the cultural method?
A: Culture methods are used to study bacteria in the field of microbiology. By using this method, we…
Q: How will you check the purity of an isolate?
A: Purity is defined as the absence of impurities in substance or the presence of matter other than…
Q: What is the Fluorochrome staining technique for Mycobacterium
A: Mycobacterium - they are a type of germ. There are different kind of mycobacterium. Still others…
Q: What is micropropagation?
A: Tissue culture technique involves growing cells or tissues in an artificial medium, which is…
Q: What is the purpose of the Ames test? How are his− bacteria used in this test?
A: Ames test is also known as Bacterial reverse mutation test. It is a method to test a chemical…
Q: What technique is used to stain bacteria? Who developed this technique?
A: Microorganisms are such small living organisms that are less than 0.1 mm, and can be seen only under…
Q: If you wanted to study this bacteria and understand what species it is, which of the following…
A: There are millions of Bacteria present throughout world. Among them some are pathogenic and and some…
Q: What is the purpose of using the streak plate method?
A: Solution- Introduction - Streak plate technique is used for the isolation into a pure culture of…
What is the methods used for identifying unknown bacteria?
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- What are Koch's postulates? Discuss the importance of these postulates in the identification of bacteriaDo we have to do an antibiotic sensitivity test to ensure the result? and what is the type and name of this bacteria?You are tasked to isolate a certain species of bacteria from a mixed culture to further characterize and identify the strain. Which technique/s is Best to perform? How shall the procedure be carried out? Explain.
- What are the advantages and limitations of studying bacteria by means of the Culture method?You've been assigned with isolating a certain species of bacteria from a mixed culture in order to characterize and identify the strain. Which technique(s) is/are the BEST to utilize? How will the procedure be carried out? Explain.Can the presence and absence of capsules in bacteria be used to identify unknown bacteria? YES OR NO? WHY?
- What is a quantitative isolation in bacterial cultivation and how it is done? Explain.What is the principle of the gel electrophoresis separation technique?a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteria