What is the difference between an enol phosphate and a normal phosphate ester that gives PEP such a high phosphoryl group transfer potential?

The enzyme prenyl transferase is responsible for condensing isopentenyl pyrophosphate units during the second stage of cholesterol biogenesis. Which of the following intermediates could still form if prenyl transferase were inhibited? (A) farnesyl pyrophosphate(B) dimethylallyl pyrophosphate (C) squalene(D) geranyl pyrophosphate(E) lanosterol

The phosphorylation and oxidative decarboxylation of oxaloacetate by inorganic phosphate (Pi) to make phosphoenolpyruvate and carbon dioxide is endergonic under intracellular conditions. It is characterized by this equation:   Oxaloacetate + Pi ←→ Phosphoenolpyruvate + H2O + CO2 ΔG’ = +24.6 kJ/mol   The synthesis of GTP from GDP and inorganic phosphate (Pi) in solution is endergonic under intracellular conditions, and it is characterized by this equation:   GDP + Pi ←→ GTP + H2O ΔG’ = +30.5 kJ/mol   Write a new net thermodynamically coupled reaction equation that describes the synthesis of phosphoenolpyruvate from oxaloacetate using the hydrolysis of GTP to power the reaction and calculate the new net ΔG’ of this reaction. Show all of your work.

A seven-residue peptide was found to have an amino acid composition including glu, val, Lys, met, leu, and trp (there is more than one copy of one amino acid). Several reactions on samples of the peptide resulted in the following: a) Trypsin has no effect on the heptapeptide. b) If one cycle of Edman degradation provides the PTH derivative shown below, what does this tell you about the oligopeptide under investigation? [image] c) Chymotrypsin treatment yields a dipeptide, a tetrapeptide, and a free amino acid. Possibilities? d) The tetrapeptide contains val, lys, and met (only). What information does this give you? e)  Cyanogen bromide treatment generates a dipeptide, a tetrapeptide, and free Lys. f)What is the sequence of the peptide?