What ionization state of Glu35 and Asp52 side chain predominates at pH 1, 4, and 7?

A one-to-one protein (P)-ligand (L) complexation (P + L PL) has a dissociation equilibrium constant (Kd) value of 100 nM at 25°C, and the Kd remains the same at 37°C. 1) What is AS of binding at 25°C? Assume ACp of the binding is 0 over the temperature range. AS = 1.34E2 kJ/(mol*K) (note the unit!!) (sig. fig =3) 2) What is the concentration of the PL complex formed at equilibrium when you mix 0.20 uM (microM) of Protein and 1.0 uM of Ligand together at 37°C? PL at equilibrium = 8.1E-1 uM (note the unit!!) (sig. fig =2)

why is non-competitive inhibiton a special type of mixed inhibiton?

If crystals of deoxy Hb are exposed to air, they break. What does this imply about the 4o structure of deoxy Hb and oxy Hb and predict which subunits junctions are most affected in the change from deoxyHb to oxyHb?