What ingredients are used in PCR?  What role does each ingredient have in replicating DNA?

Q: What are the steps of recombinant DNA technology

A: Recombinant DNA technology involves isolating, cutting, and splicing DNA from multiple organisms to…

Q: How does an RT-PCR work?

A: RT- PCR is a technique used in genetic studies used for the detection and quantification of mRNA.…

Q: What is the principle of next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: If you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR…

A: Standard PCR reagents include a set of appropriate primers for the desired target gene or DNA…

Q: Why are entire genomes not used for DNA profiling?

A: DNA profiling is the process of characterizing a sequence of DNA obtained from a person. It is also…

Q: what are the variants of PCR ?

A: PCR ( polymerase chain reaction) is a test tube method for amplifying a selected DNA sequence. There…

Q: What is the difference between the first steps of DNA sequencing to PCR in terms of the number of…

A: PCR or polymerase chain reaction is used to amplify the DNA sequence. Since DNA polymerase amplifies…

Q: How does PCR work, and why is it useful?``

A: Polymerase chain reaction (PCR) is a molecular biology technique to generate numerous DNA strands…

Q: What is a positive and negative control and what are their roles in PCR?

A: In polymerase Chain Reaction or PCR a given sample of DNA that contains very very small quantity of…

Q: What are the key differences between DNA microarrays and protein microarrays, and how they are used…

A: Introduction: A laboratory method used to recognize the expression of thousands of genes…

Q: Both cloning and PCR can be used for making copies of DNA. What is the advantage or limitation of…

A: DNA(deoxyribonucleic acid) is a double-stranded helical genetic material containing thousands of…

Q: What is PCR bias? Why is it undesirable?

A: Answer: Introduction: Polymerase chain reaction (PCR) is a process extensively used to quickly form…

Q: What is PCR amplification?

A: In the field of molecular biology, however, cloning is viewed at a genetic molecular level, where a…

Q: How do quantitative PCR (qPCR) and qualitative PCR differ?

A: Polymerase Chain reaction (PCR) is a technique to amplify the single DNA into multiple copies of its…

Q: What is PCR? why is it important for the manufacture of drugs? How is it used in forensic science?

A: In the mid  1980s and important revolutionary technique of molecular Biology PCR for polymerase…

Q: What is exome sequencing ? Why it is important ?

A: The exome has historically been described as the sequence in the genome that includes all exons of…

Q: What are the Strategies for genome sequencing?

A: The genetic material in the cells is present in the form of DNA. It contains the coded form of…

Q: why are DTT and Mg added to PCRs? what is their affect and role in there?

A: The process of obtaining multiple copies of a known DNA sequence is known as gene amplification. It…

Q: What is RNA sequencing ? Why it is important ?

A: The biochemical material that is carried from the preceding generation to the succeeding generation…

Q: What primers will be used to sequence your PCR product where are those sequences on your PCR product…

A: PCR is a technique used for amplification of DNA/gene of interest. At the end of the PCR technique…

Q: What is linear amplification PCR?

A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…

Q: What would be your experimental strategy and the lab materials required to clone the complementary…

A: Aim - To clone the complementary human gene using a mammalian gene in conjunction with PCR so, first…

Q: What do you think will happen if the melting temperatures of the PCR primers (forward and reverse)…

A: PCR (Polymerase Chain Reaction) is a technique, which is used to amplify a small fragment of DNA and…

Q: What are the criteria for “good” primers in a PCR reaction?

A: Polymerase chain reaction (PCR) is the method that is widely used to make billions of copies of a…

Q: What is genome sequencing?

A: A genome is defined as a complete set of DNA in an organism which is also known as the blueprint of…

Q: What purpose do short DNA primers serve in PCR? O They determine which sequence of DNA is copied O…

A:

Q: What are the different types of PCR and their product concepts? Site and explain the comparison…

A: Introduction :- Polymerase Chain Reaction (PCR) is a molecular biology technique for making multiple…

Q: What is the difference between whole exome and whole genome sequencing?

A:

Q: How does RT-PCR differ from traditional PCR?

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: what is   Inverse PCR ?

A: PCR which stands for polymerization chain reaction is an analytic technique which is used to amplify…

Q: How many DNA molecules would exist after 20 PCR cycles

A: Denaturation of target DNA (thermal cycle to separate DNA strands), annealing of primers to ssDNA,…

Q: What are the advantages and disadvantages of microarraytechnology compared to sequencing PCR…

A: Gene expression studies: In gene expression studies, RNA is isolated from the cell, and cDNAs are…

Q: What are sonme applications of PCR?

A: According to guidelines we have to answer the first question only. so please kindly post the…

Q: What is the difference between the first steps of DNA sequencing to PCR in terms of the number of…

A: PCR is expanded as a polymerase chain reaction. It is a technique that is used widely for the…

Q: What is the final volume of the individual PCR reactions we are making?

A: Polymerase chain reaction (PCR) is an in vitro method of making large number of copies of desired…

Q: What is recombinant DNA technology ?

A: Recombinant DNA Technology Genetic engineering is the usage of technology to alter the genome of a…

Q: What are the reagents and equipment used in PCR?

A: Polymerase Chain Reaction(PCR) is a laboratory technique introduced by Kary Mullis in 1980s to…

Q: How accurate is whole exome sequencing?

A: The goal of exome sequencing, also known as whole genome sequencing is to identify genetic variants,…

Q: what are some applications of recombinant dna technology?

A: Introduction :- The combining of DNA molecules from two distinct species is known as recombinant DNA…

Q: Why do you detect mutations one at a time on PCR? Is there any way to detect multiple mutations in…

A: PCR that is Ploymerase chain reaction is a molecular technique used in the laboratories to amplify…

Q: What is a n automated DNA sequencing?

A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…

Q: What are the advantages of PCR?

A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chain that is…

Q: What are some general “requirements” for recombinant DNA technology?

A: The general requirements for the recombinant DNA technology are the following: Sequence specific…

Q: why in PCR we use different temperature ranges in each steps?

A: Polymerase Chain Reaction (PCR) is a method used for copying DNA samples. It amplifies a small…

Q: What are the three main steps of PCR reaction? Explain them briefly

A: Biological macromolecules are large molecules that are necessary  for life in order to perform…

Q: What are the steps of a PCR?

A: PCR stands for Polymerase Chain Reaction. It is a technique of molecular biology which is used to…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.