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What are the differences between “primary cell culture” and “continuous cell lines”?.
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- Explain the expressions of primary culture, cell haH, and continuous cell haH. Explain the reason why some cells grow in suspension in culture and some grow by adhering. Give examples of these two cell types.What is a Conditioned cell-free medium(CCFM)?Why is mechanical method of cell disruption often employed even after disrupting cells with non-mechanical methods? Briefly explain some of the mechanical methods of cell disruption.
- What conditions are required to obtain a porous membrane by the phase inversion process?List and describe the three main types of cytoskeleton. If you wanted to do immunocytochemistry to specifically stain each type of cytoskeleton, what is a protein that could be used for each cytoskeletal type (in other words, what is a unique protein for each cytoskeletal type)? What are three types of actin structures? Describe the involvement of actin structures in cell migration. How is the growth and shrinking of microtubules regulated? Then describe the roles of microtubules in: chromosomal separation during mitosis and the movement of organelles and vesicles within a cell. Describe a possible mechanism on how signaling pathways might impact the cytoskeleton, so that cell migration could be regulated in a localized manner within a multicellular organism. (hint: think about the possible transcriptional regulation of the G-protein regulators) What are 2 main challenges of protein targeting? Then describe one way these challenges are overcome during signal-based targeting and one way…For which of the following apply: the extracellular space is eliminated here near the surface between two cells. A) Zonula adherens B)Zonula occluded C) Desmosome D) Gap junction E) Hemidesmosome
- You wish to identify the presence of a specific protein inside the cell. What microscopy technique should you use? a) Transmission electron microscopy b) Light microscopy Oc) Scanning electron microscopy d) Fluorescent microscopy e) None of the aboveIf the potato cells were hypotonic to the surrounding solution, the surrounding solution itself would be what to the potato cells (hypertonic, hypotonic, or isotonic)?What is the cell lysate? What is the purpose of the cheesecloth?