Chapter11: Measuring Insulin Dosages
Section: Chapter Questions
Problem 1.17P
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What are selectable markers?
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Step 1 introduction
DNA (deoxyribo nucleic acid) is the genetic material in most organisms. Recombinant DNA (rDNA) technology is the technique of manipulating the genome of a cell or organism so as to change the phenotype desirably.It is used to obtain therapeutic proteins like hormones and vaccines.
The basic steps involved in the process are:
- Isolating the DNA from the donor.
- The DNA from the donor is fragmented and screened for the desired gene.
- The gene is then inserted into the cloning vector to develop recombinant DNA.
- The recombinant DNA is then introduced into the suitable host organism by the process of transformation.
- The suitable host then creates multiple copies of the genome and also can express the desired gene.
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