Using the following data, determine the correct sequence of the octapeptide. Edmund's Reaction = PTH-Asn; • Partial Hydrolyses = o Glu, Gly; Leu, Asn, Gly; His, Met; Met, Glu; Asn, Leu; His, Val, Ser; o His, Val What is the 7th amino acid in the sequence? leucine methionine histidine valine o serine o asparagine

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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**Determining the Sequence of an Octapeptide**

Given Data for Analysis:
- **Edmund's Reaction**: PTH-Asn
- **Partial Hydrolyses**:
  - Glu, Gly
  - Leu, Asn, Gly
  - His, Met
  - Met, Glu
  - Asn, Leu
  - His, Val, Ser
  - His, Val

**Task**: Using the data provided, determine the correct sequence of the octapeptide.

**Question**: What is the 7th amino acid in the sequence?

**Options**:
- Leucine
- Methionine
- Histidine
- Valine
- Serine
- Asparagine

**Explanation of Chemical Processes**:
- **Edmund Degradation**: A method used for sequencing amino acids in peptides. The result of this reaction shows that the N-terminal amino acid is asparagine (Asn).
- **Partial Hydrolysis**: Breaking the peptide bond at several points provides small fragments, which help deduce the sequence.

**Note**: Since graphs or diagrams were not provided, no further detailed explanation of those elements is required.
Transcribed Image Text:**Determining the Sequence of an Octapeptide** Given Data for Analysis: - **Edmund's Reaction**: PTH-Asn - **Partial Hydrolyses**: - Glu, Gly - Leu, Asn, Gly - His, Met - Met, Glu - Asn, Leu - His, Val, Ser - His, Val **Task**: Using the data provided, determine the correct sequence of the octapeptide. **Question**: What is the 7th amino acid in the sequence? **Options**: - Leucine - Methionine - Histidine - Valine - Serine - Asparagine **Explanation of Chemical Processes**: - **Edmund Degradation**: A method used for sequencing amino acids in peptides. The result of this reaction shows that the N-terminal amino acid is asparagine (Asn). - **Partial Hydrolysis**: Breaking the peptide bond at several points provides small fragments, which help deduce the sequence. **Note**: Since graphs or diagrams were not provided, no further detailed explanation of those elements is required.
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