Use the given experimental data to deduce the sequence of an octapeptide that contains the amino acids Gly, Asn (2 equiv), Asp (2 equiv), Ala, Thr, and Glu. Edman degradation cleaves Asn from the octapeptide, and carboxypeptidase forms Thr and a heptapeptide. Partial hydrolysis forms the following fragments: Glu-Asp-Thr, Gly-Asp-Glu, Asn-Ala-Gly-Asp, and Asn. Be sure to answer all parts.
Use the given experimental data to deduce the sequence of an octapeptide that contains the amino acids Gly, Asn (2 equiv), Asp (2 equiv), Ala, Thr, and Glu. Edman degradation cleaves Asn from the octapeptide, and carboxypeptidase forms Thr and a heptapeptide. Partial hydrolysis forms the following fragments: Glu-Asp-Thr, Gly-Asp-Glu, Asn-Ala-Gly-Asp, and Asn. Be sure to answer all parts.
Chemistry: Principles and Reactions
8th Edition
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:William L. Masterton, Cecile N. Hurley
Chapter23: Organic Polymers, Natural And Synthetic
Section: Chapter Questions
Problem 31QAP: On complete hydrolysis, a polypeptide gives two alanine, one leucine, one methionine, one...
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