Two cultures of a facultative anaerobe are grown in the same medium, but one culture is exposed to air and the other is maintained under anaerobic conditions. Which of the two cultures will contain more cells at the end of a week? Why?
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Two cultures of a facultative anaerobe are grown in the same medium, but one culture is exposed to air and the other is maintained under anaerobic conditions. Which of the two cultures will contain more cells at the end of a week? Why?
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- E. coli, a facultative anaerobe, is grown for 24 hours on the same type of solid medium, but under two different conditions: one aerobic, the other anaerobic. The size of the colonies would bea) the same under both conditions.b) larger when grown under aerobic conditions.c) larger when grown under anaerobic conditions.Assume tu inoculated 100 facultative anaerobic cells unto nutrient agar and incubated the plate aerobically. You then inoculated 100 cells of the same species unto nutrient agar and incubated the second plate anaerobically. After incubation for 24 hours, you should theoretically have: A) More coloneis on the aerobic plate B) More colories on the anaerobic plate C) The same number of colonies on both paltes D) Fewer colonies on the anaerobic plate E) None of the aboveAssume tu inoculated 100 facultative anaerobic cells unto nutrient agar and incubated the plate aerobically. You then inoculated 100 cells of the same species unto nutrient agar and incubated the second plate anaerobically. After incubation for 24 hours, you should theoretically have: A) More coloneis on the aerobic plate B) More colories on the anaerobic plate C) The same number of colonies on both paltes D) Fewer colonies on the anaerobic plate
- a) Draw the diagram and describe motility required for the bacteria that have flagella to an attractant (light)? b) Draw the diagram of the two-chamber microbial fuel cell under continuous operation and label completely.starting with four bacterial cells per milliliter in a rich nutrient medium, with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in 1 ml of this culture after 10 hours?Why might clinical medicine have an interest in understanding bacterial cell division at the molecular level? Explain why a hyperthermophile would probably not be a human pathogen. Describe four factors that may have an influence on the effectiveness of an antimicrobial treatment. Explain why 70% or 80% alcohol is more effective than 100% alcohol in controlling microorganisms.
- You inoculate two test tubes with the same amount of identical growth medium and with the same number of identical yeast cells and grow these cells under identical conditions except for the presence or absence of oxygen. a) After 12 hours, all of the glucose in each culture has been consumed. You determine the number of total cells found in each culture and find that one culture has more cells than the other. Which culture would have the greater cell density, the one grown aerobically or the one grown anaerobically? Explain why the culture you chose above can make more cells with the same amount of glucose than the other culture.1) Listen In the lab you are testing a bacterium's oxygen requirement for growth. You grow your bacterium in a test tube, without shaking at the appropriate temperature. The next day you find that your bacterial culture is growing only at the top surface (ie at the top of the tube closest to the cap) and not dispersed throughout the test tube. What term would be used to describe the oxygen requirement of this bacterium? O A) Obligate aerobe. O B) Microaerophile. O C) Anaerobe. D) Obligate anaerobe. E) Facultative anaerobe.Two flasks of E. coli are grown in batch culture in the same medium (2% glucose and amino acids; no nitrate) and at the same temperature (378C). Culture #1 is well aerated. Culture #2 is anoxic. After 16 hours the following observations are made: ■ Culture #1 has a high cell density; the cells appear to be in stationary phase, and the glucose level in the medium is reduced to 1.2%. ■ Culture #2 has a low cell density; the cells appear to be in logarithmic phase, although their doubling time is prolonged (over 1 hour). The glucose level is reduced to 0.2%. Why does culture #2 have so little glucose remaining relative to culture #1, even though culture #2 displayed slower growth and has less biomass?
- Assume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution? helpful formula: g (generation time) = 0.301 (time)/ log x – log xoWhat is one difference between fermentation and anaerobic respiration? Since neither requires oxygen, why would a microbe select one of these versus the other? Name a specific microbe which uses fermentation and a specific microbe which uses anaerobic respiration.The generation time of a bacterium was measured at two different temperatures. Which results would be expected of a thermophile?a) 20 minutes at 10°C; 220 minutes at 37°Cb) 220 minutes at 10°C; 20 minutes at 37°Cc) no growth at 10°C; 20 minutes at 37°Cd) 20 minutes at 45°C; 220 minutes at 65°Ce) 220 minutes at 37°C; 20 minutes at 65°C