Transformation experiment was first performed on which bacteria?

Categorize the following as being associated with transformation, conjugation, or transduction. Note that some items may be associated with more than one process. Transformation Conjugation Transduction requires a virus can transfer dead bacterium's DNA to a new host cell efficient way to get naked plasmid into E. coli cells in the lab involves a pilus describes type of horizontal gene transfer in bacteria cells need to be competent process exploitod by biotechnology transfer requires at least two living bacteria alters bacterial genome

Bacterial systems serve as an excellent model to express proteins but has a disadvantage – what is that disadvantage? List the points to differentiate three classes of restriction enzymes? Give an example of restriction enzyme that has ability to generate blunt and cohesive ends after digestion of DNA?

In Cohen-Boyer’s recombinant DNA procedure, ___i___ must be used for both the bacterial DNA and the amphibian DNA ___ii___ a) the same restriction enzyme; so that the restriction sites are identical in the DNA of each species b) different restriction enzymes; So that the genes outside the restriction site are maintained c) different restriction enzymes; to ensure that the newly introduced genes are maintained in the bacterial DNA d) the same restriction enzyme; to ensure that the newly formed DNA can replicate

What type of enzymes are used to “cut” desired DNA sequences for use in recombinant gene technology experiments? Identify those two enzymes used to cut and paste both genes into the plasmid. Identify all three strategies used in this lab to maximize transformation success. Explain what it means for bacteria to be “competent.” Explains why bacterial competency is this important for this investigation.

Why do we want to genetically manipulate bacterial?   how can we genetically manipulate bacteria?   How can we tell if we have successful transaction?

Why is a thermostable form of DNA polymerase (e.g., Taqpolymerase) used in PCR? Is it necessary to use a thermostableform of DNA polymerase in the dideoxy method or in site-directedmutagenesis?

What is a reason that a cut digest insert would not match up in length with a PCR insert in gel electrophoresis?

If you had primed reverse transcription of your E. coli RNA with an oligo-dT primer rather than a random hexamer, how would that affect our experiment? (refers to the experiment Reverse transcript and RT-PCR).

What is the role of streptomycin in CRISPR experiment? What biochemical changes (DNA, protein) occurred in those cells in which CRISPR worked?