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- Pasteur’s experiments on spontaneous generationcontributed to the methodology of microbiology,understanding of the origin of life, and techniques for thepreservation of food. Explain briefly how Pasteur’sexperiments affected each of these topics.Help indetifying the unknown bacteria ? Gram Stain: Pink Cell Morpholgy: Bacillus Oxygen Requirement: OA Acid Fast Stain: green Endospore Stain: pink rods Blood agar-hemolysis: lysis (yellow clearing) Phenol red-glucose fermentation:red,no gas Phenol red -lactose fermentation:red,no gas Phenol red - mannitol fermentation:red,no gas Phenol red-sucrose fermentation: red,no gas Methyl red:no change Voges-Proskaur: no change Oxidase: purple Catalase: bubbles Nitrate Reduction: red after zinxHelp indetifying the unknown bacteria ? Gram Stain: Purple Cell Morpholgy: Rods Oxygen Requirement: OA Acid Fast Stain: pink Endospore Stain: pink rods Blood agar-hemolysis: no lysis Phenol red-glucose fermentation:red,no gas Phenol red -lactose fermentation:red,no gas Phenol red - mannitol fermentation:red,no gas Phenol red-sucrose fermentation: red,no gas Methyl red:no change Voges-Proskaur: no change Oxidase: no change Catalase: bubbles Nitrate Reduction: red after a & b
- explanation of how the pure culture of the bacteria mycobacterium ulcerans should have the ability to make the healthy host sick when skin and bone defect ,when inoculated with the cultureUse the following diagram to explain how Pasteur’s swan-necked flasks prevent contamination of sterile broth the flasks.Describe what happens to the sterile broth (a) after it has beenallowed to cool as in (b). What happens to the broth after theflask has been tipped enough to let the broth come in contactwith the dust and microorganisms and is tipped back as in (c)?What did Pasteur's results show? ► View Available Hint(s) When growth medium is not contaminated by preexisting cells, cells still grow there. When growth medium is sterilized by boiling, it remains sterile even if new cells are introduced. When growth medium is not contaminated by preexisting cells, the growth medium remains sterile. O With enough time, any sterile environment will become contaminated with cells.
- Explain the principle behind the Pasteur flask instudies on spontaneous generationThe efffectiveness of the spread plate method in microbiology.Signs and symptoms from this organism are as follows: Intense itching and a localized rash that may appear pimple-like O Bordetella pertussis O Streptococcus agalactiae O Streptococcus mutans O Sarcoptes scabiei Question 39 This organism produces dextrần which helps the pathogen form a biofilm and dent.
- explanation of how the pure culture of the bacteria, mycobacterium ulcerans should have the ability to make the healthy host sick when the skin and bone defects ,when inoculated with the culture.The _____________ is the region between the outer membrane and inner membrane of Gramnegative bacteria.Purpose Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures you have learned about during the semester. Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes You must write up your OWN dichotomous key for all the possible unknown organisms listed on above. Writing this key requires you use the same type of reasoning used in the Dichotomous key lab. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Please help me with this question. Thank you so much !