There are several feeder pathways that lead other monosaccharides into glycolysis. Below are the reactions for D-mannose: CHEMICAL EQUATION Mannose + ATP → Mannose 6-phosphate ENZYME RESPONSIBLE AG° (kJ/mol) hexokinase -15.7 phosphomannose isomerase +2.5 Mannose 6-phosphate → Fructose 6-phosphate The AG° values for the full glycolytic pathway can be found in the following table. The reported values are for ONE MOLE of the appropriate reactant being converted into product. Step AG (/mol) Step AG" (/mol) 1 -16.7 6 +6.3 2 +1.7 7 -18.5 3 -14.2 8 +4.4 4 +23.8 9 +7.5 5 +7.5 10 -31.4 مل

There are several feeder pathways that lead other monosaccharides into glycolysis. Below are the reactions for D-mannose: CHEMICAL EQUATION Mannose + ATP → Mannose 6-phosphate ENZYME RESPONSIBLE AG° (kJ/mol) hexokinase -15.7 phosphomannose isomerase +2.5 Mannose 6-phosphate → Fructose 6-phosphate The AG° values for the full glycolytic pathway can be found in the following table. The reported values are for ONE MOLE of the appropriate reactant being converted into product. Step AG (/mol) Step AG" (/mol) 1 -16.7 6 +6.3 2 +1.7 7 -18.5 3 -14.2 8 +4.4 4 +23.8 9 +7.5 5 +7.5 10 -31.4 مل

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter18: Glycolysis

Section: Chapter Questions

Problem 2P: Radiotracer Labeling of Pyruvate from Glucose Determine the anticipated location in pyruvate of...

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The glucose/glucose-6-phosphate substrate cycle involves distinct reactions of glycolysis and gluconeogenesis that interconvert these two metabolites. Assume that under physiological conditions, [ATP] = [ADP]; [P;] = 1 mM. Consider the glycolytic reaction catalyzed by hexokinase: ATP + glucose ADP + glucose-6-phosphate AG = - 16.7 kJ/mol (a) Calculate the equilibrium constant (K) for this reaction at 298°K, and from that, calculate the maximum [glucose-6-phosphate]/ Iglucose] ratio that would exist under conditions where the reaction is still thermodynamically favorable. (b) Reversal of this interconversion in gluconeogenesis is catalyzed by glucose-6-phosphatase: glucose-6-phosphate + H20 = glucose + P AG" = -13.8 kJ/mol
Draw OUT THE arrow pushing reaction mechanism for the following steps of glycolysis: SHOW/IDENTIFY the arrow pushing mechanism in at least 2 steps. CH,OPO CH,OH hexokinase 2+ H H H H H H ОН + ATP OH H ADP + H+ H ОН + НО HO OH H ОН H ОН Glucose-6-phosphate (G6P) Glucose
The glucose/glucose-6-phosphate substrate cycle involves distinct reactions of glycolysis and gluconcogenesis that interconvert these two metabolites. Assume that under physiological conditions, [ATP] = [ADP] and [Pi] =1 mM. Consider the following glycolytic reaction catalyzed by hexokinase: ATP + glucose = AG' = -16.7 kJ/mol ADP + glucose-6-phosphate (a) Calculate the equilibrium constant (K) for this reaction at 298 K, and from that, calculate the maximum [glucose-6-phosphate]/[glucose] ratio that would exist under conditions where the reaction is still thermody- namically favorable. (b) The reverse of this interconversion in gluconeogenesis is catalyzed by glucose-6-phosphatase: glucose-6-phosphate + H,0 = glucose + P, AGr = -13.8 kJ/mol K= 262 for this reaction. Calculate the maximum ratio of [glucose]/ [glucose-6-phosphate] that would exist under conditions where the reaction is still thermodynamically favorable. (c) Under what cellular conditions would both directions in the…
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