The volume of sample loaded (Vinj) to a capillary by hydrodynamic injection can be calculated using the Hagen-Poiseuille equation, 4 Vinj = = [T d tini API(128n L) where AP = pressure difference across the capillary, d = capillary inside diameter, tini = injection time, n = buffer viscosity, L = total capillary leng What volume of sample in nanoliter (nL) would be applied to a 50 um ID x 20 cm long capillary if a pressure of 0.5 psi is applied for 1 s to a solution with a viscosity of 0.01 poise (P)? (1 psi = 6.9 x 10° Pa, 1 P = 0.1 Pa · s) O A. 0.26
The volume of sample loaded (Vinj) to a capillary by hydrodynamic injection can be calculated using the Hagen-Poiseuille equation, 4 Vinj = = [T d tini API(128n L) where AP = pressure difference across the capillary, d = capillary inside diameter, tini = injection time, n = buffer viscosity, L = total capillary leng What volume of sample in nanoliter (nL) would be applied to a 50 um ID x 20 cm long capillary if a pressure of 0.5 psi is applied for 1 s to a solution with a viscosity of 0.01 poise (P)? (1 psi = 6.9 x 10° Pa, 1 P = 0.1 Pa · s) O A. 0.26
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Transcribed Image Text:The volume of sample loaded (Vini) to a capillary by hydrodynamic injection can be calculated using the Hagen-Poiseuille equation,
4
Vinj = = [7 d tinj AP(1287 L)
where AP = pressure difference across the capillary, d = capillary inside diameter, tini = injection time, n = buffer viscosity, L = total capillary length.
What volume of sample in nanoliter (nL) would be applied to a 50 um ID x 20 cm long capillary if a pressure of 0.5 psi is applied for 1 s to a
solution with a viscosity of 0.01 poise (P)? (1 psi = 6.9 x 10° Pa, 1 P = 0.1 Pa · s)
O A. 0.26
О В. 1.3
O C. 1.3 × 10-3
O D. 2.6
O E. 2.6 x 10-3
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