The unicellular fungus Saccharomyces cerevisiae is used in the food industry to ferment sugars. The rate of fermentation is measured by CO2 production. The addition of inorganic phosphate increases this rate. 1. Why does the fermentation of sugars, such as glucose and fructose, depend upon the presence of Pi? Which glycolytic reactions require Pi?  2. Several glycolytic intermediates contain phosphate. Where did these groups come from during the reactions? How much Pi is required to generate one CO2?  3.  Is CO2 generation the most reliable way to measure ethanol fermentation? Why or why not?

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter19: The Tricarboxylic Acid Cycle
Section: Chapter Questions
Problem 22P: Study Figure 19.18 and decide which of the following statements is false. Pyruvate dehydrogenase is...
icon
Related questions
Question

The unicellular fungus Saccharomyces cerevisiae is used in the food industry to ferment sugars.
The rate of fermentation is measured by CO2 production. The addition of inorganic phosphate
increases this rate.

1. Why does the fermentation of sugars, such as glucose and fructose, depend upon the
presence of Pi? Which glycolytic reactions require Pi? 
2. Several glycolytic intermediates contain phosphate. Where did these groups come from
during the reactions? How much Pi is required to generate one CO2? 
3.  Is CO2 generation the most reliable way to measure ethanol fermentation?
Why or why not?

Lactate
C/DD
Lactate dehydrogenase
NAD+
Acetyl-CoA
synthetase
NADH + H
NAD
NADH + H
Pyruvate
dehydrogenase
LTDUD
AMP
+ PP
ATP
+ CoA
NADH+H.
COA
Glucose
NAD+
CO₂
Pyruvate
COA
Glycolysis
Phospho-
transacetylase
Acetyl-CoA
Pyruvate
formate lyase
Formate
Acetate
kinase
P₁
COA
Acetyl-phosphate
ADP
ATP
NADH
Acetate
Aldehyde
dehydrogenase
NAD
Acetaldehyde
NADH + H
COA
NAD+
Alcohol
dehydrogenase
Ethanol
ADHE
Transcribed Image Text:Lactate C/DD Lactate dehydrogenase NAD+ Acetyl-CoA synthetase NADH + H NAD NADH + H Pyruvate dehydrogenase LTDUD AMP + PP ATP + CoA NADH+H. COA Glucose NAD+ CO₂ Pyruvate COA Glycolysis Phospho- transacetylase Acetyl-CoA Pyruvate formate lyase Formate Acetate kinase P₁ COA Acetyl-phosphate ADP ATP NADH Acetate Aldehyde dehydrogenase NAD Acetaldehyde NADH + H COA NAD+ Alcohol dehydrogenase Ethanol ADHE
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 4 steps

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning