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The prep team for our course has prepared a 0.1 mg/mL stock of BPB solution. To get an absorbance reading near 1 on our spectrophotometer, we need to make a 12.5 µg/mL solution.

The prep team for our course has prepared a 0.1 mg/mL stock of BPB solution. To get an absorbance reading near 1 on our spectrophotometer, we need to make a 12.5 µg/mL solution.

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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2. The prep team for our course has prepared a 0.1 mg/mL stock of BPB solution. To get an absorbance reading near 1 on our spectrophotometer, we need to make a 12.5 µg/mL solution. We need 1 mL of this 12.5 ug/mL BPB for our experiment. How many microliters of 0.1 mg/mL BPB solution and how many microliters of water must we combine? Show your work below. Pay attention to your units!
Transcribed Image Text:2. The prep team for our course has prepared a 0.1 mg/mL stock of BPB solution. To get an absorbance reading near 1 on our spectrophotometer, we need to make a 12.5 µg/mL solution. We need 1 mL of this 12.5 ug/mL BPB for our experiment. How many microliters of 0.1 mg/mL BPB solution and how many microliters of water must we combine? Show your work below. Pay attention to your units!
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