The orientation of the promoter dictates the direction of transcription, and this determines which strand is used as a template. Template strand Terminator Terminator 5' 3' DNA 5' 3' Promoter 1 Promoter 2 Template strand RNA 3' 5' 5' 13' RNA FIGURE 7.11 The Promoter Orients RNA Polymerase The orientation of RNA polymerase determines which strand will be used as the template. In this diagram, the color of the RNA molecules indicates which DNA strand was used as the template. The light blue RNA was transcribed from the red DNA strand (and is therefore analogous in sequence to the blue DNA strand), whereas the pink RNA was transcribed from the blue DNA strand (and is therefore analogous in sequence to the red DNA strand).

If the following nucleotide sequence, CTC/TGT/AAG/ACC/TTT experienced a mutation resulting in the deletion of the second cytosine in the first DNA triplet so the sequence is now CT_/TGT/AAG/ACC/TTT, what would be the amino acid sequence created from this mutated DNA strand? Table of mRNA codons UUA, UUG = leucine AGG, AGA = arginine %3D CAU, CAC = histidine GUU, GÜC, GUA = valine GAA. GAG=glutamic acid GCU, GUA, GUG = alanine GAU, GAC = asparagine GGU, GGC, GGA = glycine UCA, UCU =serine CGU, CGC, CGA = arginine

What chemical group (phosphate group, hydroxyl group, or anitrogenous base) is found at the 3′ end of a DNA strand? Whatgroup is found at the 5′ end?

Where is the substitution indicated on the original DNA strand? May you please Write it out and bold the nucleotide.

If you have a strand of DNA that is GAGATCTCGC , what is the complimentary strand?

(a) Write the DNA double strand. (b) Assuming the gel pattern represents the template strand, transcribe and translate the DNA. (c) Write the anticodon sequence. A G 2nd (middle) Base of a Codon 1* 3rd U A G Base Base UUU - Phe U UUC - Phe UCU - Ser UCC - Ser UAU - Tyr UAC - Tyr UGU - Cys UGC - Cys UUA - Leu UCA- Ser UAA - STOP UGA - STOP UUG -Leu CUU - Leu CUC - Leu UCG-Ser CCU - Pro CCC- Pro UAG- STOP CAU - His САC - His UGG- Trp CGU - Arg CGC - Arg CGA - Arg CGG- Arg CỦA - Leu ССА-Pro CAA - Gin CAG- Gin AAU - Asn CUG - Leu CCG-Pro AUU - Ile ACU - Thr АCC - Th ACA - Thr AGU – Ser A AGC - Ser AGA - Arg AGG - Arg GGU - Gly GGC - Gly GGA - Gly GGG - Gly AUC - lle AAC - Asn AAA - Lys AAG - Lys GAU - Asp GAC - Asp GAA - Glu AUA- lle AUG - Met ACG - Thr G GUU - Val GCU - Ala GCC - Ala GUC - Val GUA- Val GCA - Ala GUG - Val GCG - Ala GAG - Glu PUAGPCAGUCACUCAG |