The kinetics of an enzyme are measured as a function of [S] in the presence and absence of 2 mM I. Compute Km and Vmax in the absence and presence of I   S] (uM) V (uM/min)   without I with I 3 10.4 4.1 5 14.5 6.4 10 22.5 11.3 30 33.8 22.6 90 40.5 33.8

11. Applying the Michaelis-Menten Equation III Aresearch group discovers a new version of happyase, which they call hap- pyase*, that catalyzes the chemical reaction HAPPY = SAD. The researchers begin to characterize the enzyme. (a) In the first experiment, with [E] at 4 nM, they find that the Vnux is 1.6 M s, Based on this experiment, what is the kat for happyase*? (Include appropriate units.) (b) In another experiment, with [E] at 1 nM and [HAPPY] at 30 LM, the researchers find that V, = 300 IM s. What is the measured K of happyase* for its substrate HAPPY? (Include appropriate units.) (c) Further research shows that the purified happyase* used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase* preparation and the two experiments repeated, the measured Vmax in (a) is in- creased to 4.8 uM S, pIM. For the inhibitor ANGER, calculate the values of a and o (d) Based on the information given…

Use the data below to determine the Michaelis-Menton constant [in mM] of a certain enzyme-catalyzed reaction.   v = 0.152 mM/s at [S] = 0.334 mM v = 0.176 mM/s at [S] = 0.450 mM

question 19