Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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please solve question 17

The graph below represents one of the enzymes inhibition mechanism, answer the following:
Vex (C)
A
Y
==
0
B
+1
Slope = (C)
KM
Vmax
-1
Slope =
KM
Vmax
X
Q13) Which mode of inhibition does the graph represent?
A) Pure competitive
B) Mixed competitive
C) Pure noncompetitive
D) Uncompetitive
E) Irreversible inhibition
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Transcribed Image Text:The graph below represents one of the enzymes inhibition mechanism, answer the following: Vex (C) A Y == 0 B +1 Slope = (C) KM Vmax -1 Slope = KM Vmax X Q13) Which mode of inhibition does the graph represent? A) Pure competitive B) Mixed competitive C) Pure noncompetitive D) Uncompetitive E) Irreversible inhibition
Q14: What do X and Y axis represent?
A) X axis is 1/[S] and Y axis is 1/[Vmax]
B) X axis is 1/[Vmax] and Y axis is 1/[S]
C) X axis is 1/[KM] and Y axis is 1/[V]
D) X axis is 1/[S] and Y axis is 1/[V]
E) X axis is 1/[V] and Y axis is 1/[S]
Q15: What does the point A on the X axis represent?
A) -1/[KM]
B) 1/[KM]
C) 1/[Vmax]
D) -1/KM (1+[I]/K₁)
E) KM/Vmax (1+[I])
Q16: What does the point B on the Y axis represent?
A) 1/[KM]
B) 1/[S]
C) 1/[Vmax]
D) 1/ KM (1+[I]/K₁)
E) KM
Q17: What is the factor C?
A) 1/[KM]
B) 1+[S]
C) 1+[V]
D) 1+([I]/K₁)
E) [I]/KM
Q18: 4. An ------ ----- is used to elute proteins attached to a hydrophobic interaction chromatography
in order of hydrophobicity:?
A) Increasing salt gradient
B) Decreasing salt gradient
C) Increasing pH gradient
D) Decreasing pH gradient
E) Constant salt concentration
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Transcribed Image Text:Q14: What do X and Y axis represent? A) X axis is 1/[S] and Y axis is 1/[Vmax] B) X axis is 1/[Vmax] and Y axis is 1/[S] C) X axis is 1/[KM] and Y axis is 1/[V] D) X axis is 1/[S] and Y axis is 1/[V] E) X axis is 1/[V] and Y axis is 1/[S] Q15: What does the point A on the X axis represent? A) -1/[KM] B) 1/[KM] C) 1/[Vmax] D) -1/KM (1+[I]/K₁) E) KM/Vmax (1+[I]) Q16: What does the point B on the Y axis represent? A) 1/[KM] B) 1/[S] C) 1/[Vmax] D) 1/ KM (1+[I]/K₁) E) KM Q17: What is the factor C? A) 1/[KM] B) 1+[S] C) 1+[V] D) 1+([I]/K₁) E) [I]/KM Q18: 4. An ------ ----- is used to elute proteins attached to a hydrophobic interaction chromatography in order of hydrophobicity:? A) Increasing salt gradient B) Decreasing salt gradient C) Increasing pH gradient D) Decreasing pH gradient E) Constant salt concentration
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