The following images show a pure culture that has been grown on a brewers agar deep, tested for the presence of oxidase and tested for the presence of catalase. Based on the test results can you conclude which oxygen requirement category does this organism belong to? OD LDrysde ) rewen Agar rewers Ager id Oidase test 4 Catalase test with added nitrate facultative aerobe O strict anaerobe aerotolerant aerobe facultative anaerobe aerotolerant anaerobe

The following images show a pure culture that has been grown on a brewers agar deep, tested for the presence of oxidase and tested for the presence of catalase. Based on the test results can you conclude which oxygen requirement category does this organism belong to? OD LDrysde ) rewen Agar rewers Ager id Oidase test 4 Catalase test with added nitrate facultative aerobe O strict anaerobe aerotolerant aerobe facultative anaerobe aerotolerant anaerobe

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

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Why does the oxidase test must be performed from blood agar or another medium without a fermentable sugar as MacConkey agar?
1 H 2 176 old culture Smear too BOTERC Losk like? + b. Failure to apply the decolorizer. + ne c. Failure to apply the safranin. Determine whether bacteria are motle or non-motle by looking at the growth in semi-solid medium What is the concentration of agar in (a) typical solid medium (b) semi sold motility medium? Arawer Q1 on Page 357. 7. Orygen Requirement: + d. Reversal of crystal violet and safranin stains. A questions 1.2 and 3 o Pg 83-84 Refer to the power point Questions 1 Predict the effect of the following "mistakes" made when performing a Gram stain. Your answer needs to address the predicted effect on both Gram-positive and Gram-negative cells. Consider each mistake independently. a. Failure to add the iodine. 414 gait to fer. 2 Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram-positive and Gram-negative cells. This being the case, explain how they stain different cell types in the Gram stain. why? a cell wall. patem in…
Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.
Methyl red test does the color of the medium change to red when methyl red was added? Is it positive or negative for mixed acid fermentation?
CITRATE TESTThis test determines the ability of bacteria to use citrate as source of carbon andinorganic ammonium salt as a source of nitrogen. The following are the stepwise procedurein conducting citrate test:1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needleto a colony.2. Incubate at 37 degree Celsius for 24 to 48 hours.A positive result is indicated by the pH indicator, bromothymol blue which turns the growthgreen to blue. Task: In a tabulated form, determine if the Pseudomonas aeruginosa, are positive or negative withcitrate test. Interpret your answer and include the reaction of organism on this test.
CITRATE TESTThis test determines the ability of bacteria to use citrate as source of carbon andinorganic ammonium salt as a source of nitrogen. The following are the stepwise procedurein conducting citrate test:1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needleto a colony.2. Incubate at 37 degree Celsius for 24 to 48 hours.A positive result is indicated by the pH indicator, bromothymol blue which turns the growthgreen to blue. Task: Answer the following In a tabulated form, determine if the Salmonella typhi, are positive or negative withcitrate test. Interpret your answer and Include the reaction of organism on this test.
What are the advantages of the API 20E test compared to regular biochemical tubemedia
CITRATE TESTThis test determines the ability of bacteria to use citrate as source of carbon andinorganic ammonium salt as a source of nitrogen. The following are the stepwise procedurein conducting citrate test:1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needleto a colony.2. Incubate at 37 degree Celsius for 24 to 48 hours.A positive result is indicated by the pH indicator, bromothymol blue which turns the growthgreen to blue. Task: In a tabulated form, determine if Salmonella typhi the are positive or negative withcitrate test. Interpret your answer and include the reaction of organism on this test.
From figure shows triplesugar iron agar. What biochemical characteristics does this figureillustrate? How could this medium be used to begin the identificationof the isolated bacteria in this chapter’s Case Study?
Can you classify the following biochemical tests based on the following criteria and explain why you did so? Test based on the presence of extracellular enzymes Test based on the presence of intracellular enzymes Test ased on carbohydrate fermentation Test based on protein catabolism 1. Catalase Test 2. Oxidase test 3. MacConkey agar (MAC) Medium Test 4. Mannitol Salt Agar (MSA) Medium Test 5. Gelatin Hydrolysis 6. Indole Production 7. Motility-Indole-Ornithine Decarboxylation Test 8. Methyl Red / Voges-Proskauer (MR/VP) Test 9. Nitrate Reduction Test 10. Simmon’s Citrate Agar Slant 11. Triple Iron Sugar Test 12. Urease Test
In the tripple sugar fermentation test, why is the triple sugar iron agar called a multi-test system? list the concentrations of sugars in the media and the possible results due to fermentation
Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.