TEST I. Fill in the necessary concepts about microscopes. OF COMPounb dissection or stereoscope SCAnning ELECTRON TYPES MICROSCOPE MICROSCOPE micRoscoPE (SEM) THAT APPEARS The image is seen in The image seen THE iMAGE with this type of In THIS TYPE OF MICROSCOPE 3-D. microscope is two dimensional. Description IS THREE DIMENSIONAL. This microscope is the commonly most used. visible light ELECTRONS Source of Radiation VISIBLE UGHT for Image Formation Medium AIR VACUUM air light scattering or light ELECTRON reflection Means of LIGHT Major Providing specimen Contrast SCATTERING ABSORPTION Resolution low low Magnification
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- Please help using the image i attached below. There are 3 parts to this question. Describe how this is related to light scattering. Note the field is always dark in this type of microscope (and hence, called dark-field). Why is this? Explain how this type of microscope can be used to detect (but not resolve) flagella.1. Identify features of Dark-field Microscopy (1-2 paragraph) 2. Give three examples of the uses of microscopes outside the field of biology and medicine. (3 examples with 5sentences explanation each)answer the following: instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:
- SBI 3C1 VIRTUAL LAB: THE MICROSCOPE INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the instructions on how to properly use a microscope. When you are complete, answer the questions below. PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen? TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification) Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE ОBJECTIVE LENS POWER OCULAR MAGNIFICATION OBJECTIVE LENS MAGNIFICATION TOTAL MAGNIFICATION MAG (X) = Ocular X Objective LOW LP MAG = MEDIUM MP MAG= HIGH HP MAG- Complete the following chart by calculating the missing lens or total magnification [2] TOTAL MAGNIFICATION OBJECTIVE LENS MAGNIR AR (EYEPIECE) MAGNIFICATION 5X 80X 10X 40X 10X 100X 500X 50X PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES Access the Virtual Microscope at…Analysis: Write your answers on the space provided. 1. How does the letter “e” as seen through the microscope differ from the way an “e” normally appears? 2. When you move the slide to the left, in what direction does the letter “e” appear to move? When you move it to the right? Up? Down? 3. How does the ink appear under the microscope compared to normal view? 4. Why does a specimen placed under the microscope have to be thin?1.Why is wavelenth the main limiting factor on limit of resoltuin in light microscopy? 2.Assuming that all other variables remain constant, explain why light of shorter wavelengths will produce a clearer image that light of longer wavelenths. 3. Why aren't the magnification of both ocular lenses of a binocular microscope used to calculate total magnification?
- L of Microscopes: 1. If you have a standard ocular lens of 10x what is the true magnification that you are looking at if you are using the 100x objective lens. What is the rule for focusing if you switch to the 40x lens? 2. Calculate the sizes of the following cells. Not all cells are viewed at 40x so use the following table to calculate the size of your cells. 4x 1 reticular unit = .025 mm 10x 1 reticular unit = .01 mm 40x 1 reticular unit-.0025 mm 4 points A) Cell 1 on 10x=8 reticular units B) Cell 2 on 40 x = 28 reticular units mm mm Which one is actually larger? Which one is probably the plant cell? Why?Microscope Structure and Function Compound Light Microscope Structures: Label the names of the microscope components (structures) on the diagram below. Some structures have multiple correct names. 1. 8. 2. 3. 9. 4. 10. 5. 11. 6. 7.Describe the operating features of electron microscopes andhow they differ from optical microscopes in illumination source,magnification, resolution, and image appearance.
- Compare and contrast the operation of Optical microscopy and TEM in terms of Contrast (explain all the different types for each microscopy)What 3 simple ways of preparing microscope slides for light microscopy..The magnification possible with any type of microscope is an important property, but its resolution, the ability to distinguish between two very closely apposed objects, is even more critical. Describe why the resolving power of a microscope is more important for seeing finer details than its magnification.