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- Indicate whether the following compounds is positive or negative in the Benedict’s test: 1. Sucrose hydrolysate 2. Starch hydrolysate 3. Cellulose hydrolysateQualitative Analysis of Carbohydrates HELP Select the test: Glucose, lactose and sucrose are soluble in water, Solubility test whereas starch is insoluble in water. Water Reset Glucose Lactose Sucrose Starch A,We did an experiment about carbohydrate chemistry and the prof did not elaborate on the details. She just gave the positive result and reagent. I would like to ask the following: Kindly explain the mechanism/principle of the Benedict's test that occurred with the samples (enumerated) Give the chemical equation for each. Glucose Sucrose Ribose
- BARFOED’S TEST FOR MONOSACCHARIDESPlace 1 mL (20 drops) of each 1% carbohydrates solution in separate test tubes. Prepare a control with 1 mL water. Add 4 mL Barfoed’s reagent. Place the test tubes in boiling water bath for 10 minutes. Remove the test tubes and cool in cold water. Record your observations. A precipitate of cuprous oxide which may be green to red, indicates that a monosaccharide is present. Record your result and conclusion. Identify the compounds that are monosaccharides. SELIWANOFF’S TEST FOR KETOHEXOSESPlace 5 drops of 2% carbohydrate solution in separate test tubes. Prepare a control with 5 drops water. Add 1 mL of Seliwanoff’s reagent to each test tubes and heat. Record your results. The rapid formation of a bright red indicates that a ketohexose is present. Identify the compounds that are ketohexoses. IODINE TESTS FOR POLYSACCHARIDESPlace 2 mL of each carbohydrate solution in separate test tubes. Prepare a control with 2 mL of water. Add 1 drop of iodine solution…Which Unknown (a, b, c, d, or e) matches the test results pictured here (same as previous question)? Sadan Unkdwin Carbohydrate Sugar total (g) Fiber Unknown (g) (g) Protein Fat 25 25 50 20 30 16 15 65 2 21 12 d. 75 75 26 e 15 70 1 Biuret Lodine BesedictsQualitative Analysis of Carbohydrates HELP Select the test: Place test tubes in waterbath Fehling's test A Fehling' solution Fehling's solution Reset Glucose Lactose Sucrose Starch Qualitative Analysis of Carbohydrates HELP Reducing sugars (glucose & lactose) reduce the deep blue solution of copper (II) in the test reagent to red precipitate of insoluble cuprous oxide. Select the test: Fehling's test Reset
- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Molish ’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.Qualitative and Quantitative Tests for Amino Acids and ProteinsComplete the table: Test Reagents Positive Result Positive Indication Ninhydrin test Xanthoproteic acid test Millon's test Hopkins Cole test Pauly's diazo Test Lead sulphide test Histidine test Isatin test Folin’s McCarthy Sullivan Test Nitroprusside TestTrue or False Galactose gives a positive result with Benedict’s test faster than lactose due to the acidity of the reagent used.
- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Benedict ’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (this is not a graded question)Write a description of the physical characteristics of the isolated starch and glycogen. Provide the observable result after subjecting the said intact polysaccharides in Molisch’s test and Iodine solution. Intact Sample Physical Characteristics Molish’s Test I2 solution Starch GlycogenDESCRIBE THE RESULTS OF THE REDUCTION TEST FOR SUGARS USING THE FOLLOWING TEST PICRIC ACID TEST SUGAR RESULT 1. Glucose 2. Sucrose 3. Maltose 4. Fructose 5. Lactose 6. Galactose