Table 27.2 pK, Values for lonizable Groups of Amino Acids pK of a-COOH pK, of a-NH, pK, of Side Chain Isoelectric Amino Acid Point (pl) Alanine 2.35 9.87 6.11 Arginine 2.01 9.04 12.48 10.76 Asparagine 2.02 8.80 5.41 Aspartic acid 2.10 9.82 3.86 2.98 Cysteine 2.05 10.25 8.00 5.02 Glutamic acid 2.10 9.47 4.07 3.08 Glutamine 2.17 9.13 5.65 - Glycine 2.35 9.78 6.06 - Histidine 1.77 9.18 6.10 7.64 Isoleucine 2.32 9.76 6.04 Leucine 2.33 9.74 6.04 Lysine 2.18 8.95 10.53 9.74 Methionine 2.28 9.21 5.74 Phenylalanine 2.58 9.24 5.91 Proline 2.00 10.60 6.30 Serine 2.21 9.15 5.68 Threonine 2.09 9.10 5.60 Tryptophan 2.38 9.39 5.88 Tyrosine 2.20 9.11 10.07 5.63 Valine 2.29 9.72 6.00

Q: Given pKa values for ionizable groups from Table 27.2, sketch curves for the titration of Q.)…

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Q: You have been given a mixture of lysine, histidine and cysteine. The isoelectric point of the amino…

A: The mixture of amino can be separated by electrophoresis technique. This method is based on pH…

Q: indicate which of the amino acid residues in the following peptide sequence contains a group that…

A: When the carboxyl group of one molecule reacts with the amino group of the other molecule, releasing…

Q: lonization State of Histidine Each ionizable group of an amino acid can exist in one of two states,…

A: “Since there are multiple questions and it is not mentioned that which one has to be solved so I am…

Q: amino acid will elute first

A: For cation exchange column, pH is kept slightly lower in comparison to pI value so that the…

Q: Each ionizable group of an amino acid can exist in one of two states, charged or neutral. The…

A: Histidine has the three ionizable functional groups and we have to assign proper pKa and net charge…

Q: All amino acids have two ionizable functional groups: an a-amino group (average pK, of 9.4) and an…

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Q: Which amino acids would be expected to produce a similar sickling effect if substituted for Val at…

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Q: Consider a peptide: Glu-Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly-His If the pKa values for the…

A: If the pH of the solution is greater or equal to the pKa of side chain then the side chain will be…

Q: Draw the chemical structure of the following amino acid sequence at pH 7, label the peptide bonds,…

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Q: 5 ased on molecular weights of purified (no beta-Me) and (beta-Me) sample, which are 97.4 kDa and…

A: Given , molecular weight of (no beta -Me ) purified sample = 97.4 kDa molecular weight of (beta -Me)…

Q: A given glycine solution was titrated against NaOH and pH of the final solution was 4.8. Given that…

A: Given amino acid is glycine.  pKa1 is given as 2.34 and pKa2 is given as 9.60.  Concentration of…

Q: The maximum number of amino acid side chains that can potentially form an ionic bond (+lil-…

A: Histidine is an amino acid with a imidazole ring attached as a substituent. The polarity of…

Q: Use three-letter amino acid abbreviations to give the sequence of amino acids in this polypeptide.…

A: Answer:- This question is answered by using the simple concept of naming of a polypeptide using the…

Q: draw the primary structure of the peptide DKMMS at pH 7.4 and name this peptide and what chain…

A: Primary structure of a peptide refers to the amino acid sequence in the peptide.

Q: As shown in Mechanism 29.2, the nal steps in the Edman degradation result in rearrangement of a…

A: The given compound thiazolinone is less stable than N-phenyl thiohydantoin and undergoes…

Q: 26. Almost all proteins are composed from a set of about amino acids. 20 d. 50 a. 4 27. a. 5 b. 10…

A: 1. Almost all proteins are composed from a set of about  20  amino acids. 2. 10 amino acids are…

Q: The peptide cys-leu-glu-ala-cys-lys is at pH 7 in oxidizing conditions. Part a) Draw the peptide's…

A: The given peptide is cys-leu-glu-ala-cys-lys. Given pH = 7

Q: Which representation of the amino acid lysine would exist at a pH less than the isoelectric point?…

A: Given amino acid,

Q: The isoelectric point (pl) of a peptide is the pH at which the Group pK peptide ceases to migrate in…

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Q: In the tetrapeptide Phe-Gln-Trp-His, the C-terminal amino acid is A. Phe B. Trp C. His D. Gln

A: whenever a peptide is drawn or written left amino acid represent N-terminal amino acid like here Phe…

Q: This is a three-part question about a tripeptide. Answer all three sub-questions. The pKR of side…

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Q: which amino acids has a side chain that can serve as a hydrogen bond donor but not acceptor in its…

A: Hydrogen bond donor is the one which can donate hydrogen atom to form hydrogen bonding.  Hydrogen…

Q: You have been given a mixture of lysine, histidine and cysteine. The isoelectric point of the amino…

A: Electrophoresis- Separation of charged particles with the help of electric field. It consists of…

Q: What type of interaction (IMF) would be present between side chains of the following amino acid…

A: If anything in this world occupies any space then it will be considered as the Matter. Generally,…

Q: Consider the pentapeptide Ile-Lys-Asp-Phe-Gly and answer the following (use the pKa values in the…

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Q: (a) Describe in detail how you will determine the primary structure of protein. (b)You have been…

A: The primary structure of a protein is generally referred to the sequence of amino acids in the…

Q: The amino acid sequence shown below is a short excerpt (residues 130-172) from the rod domain of an…

A: Following the rules of conservative amino acids, the amino acids that when replaced with a…

Q: Given the following peptideSEPIMAPVEYPK(a) Estimate the net charge at pH 7 and at pH 12. Assume the…

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Q: 17. H,N-CH-C-NH-CH, -COOH À BC D Ė Where is the peptide linkage in the dipeptide above? a. A Ob. B…

A: A peptide bond is a chemical bond formed between two molecules when the carboxyl group of one…

Q: Consider the peptides below. HO OH Peptide 1: HO. HO. HO, Peptide 2: pro-ile-glu-arg How many…

A: Amino acids can be defined as the organic compound containing an amino group and carboxylic group…

Q: Glycine solution was titrated against NaOH and pH of the final solution was 4.8. Given that the pKa¹…

A: Given data :  Glycine solution was titrated against NaOH and pH of the final solution was 4.8.…

Q: Proteins A, B, and C have isoelctric points of 8.2, 6.7,  and 5.2 respectively.  Place proteins A,…

A: The heavy molecules travel shorter distance whereas light molecules travel larger distance under the…

Q: The compound shown below is used to fluorescently label proteins: но. но Which of the following…

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Q: The amino acid (S)-alanine has the physical characteristics listed under the structure. What is the…

A: Enantiomers are the stereoisomers which are nonsuperimposable mirror image of each other. All the…

Q: Determine the amino acid sequence of a polypeptide from the following data: Complete hydrolysis of…

A: We can use the above given info to write the primary structure of the original polypeptide. First we…

Q: Indicate which of the amino acid residues in the following peptide sequence contains a group that…

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Q: Use three-letter amino acid abbreviations to give the sequence of amino acids in this polypeptide.…

A: To write the name of the given polypeptide, first of all we write the name of the amino acids by…

Q: What is the predominant form of the following amino acids at pH= 1? What is the overall charge on…

A: At isoelectric point, the amino acids exist in neutral form. The amine groups exist as -NH3+ and…

Q: identify the amino acid described. state the name, one-letter abbreviation, and draw the structure…

A: We have to identify the amino acid described. state the name, one-letter abbreviation, and draw the…

Q: Oxoglutarate can be used to generate which of the following amino acids? ASPGR

A: Answer

Q: Write the structure of all possible peptides containing these amino acids: Ala, Leu, Use single…

A: The abbreviations for the given amino acids are: Alanine      Ala    ALeucine      Leu    L

Q: For aspartic and glutamic acids, the isoelectric point occurs at a pH where the net charge on the…

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Q: Aspartamen, which is commonly known as Nutrasweet contain the followin dipetide a. what are the…

A: The aminoacids present in aspartame has to be given.

Q: Draw the tetrapeptide Phe-Pro-Met-Gln. Put an X on all the peptide bonds. Circle all the chirality…

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Q: What is the pI for the polypeptide ALRHEN? Use pKa of 8 for the N-terminus and 4 for the C-terminus

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Q: A compound is known to have a free amino group with a pKa of 8.8, and one other ionizable group with…

A: Given compound is having two ionisable groups.

Q: What is the percentage of the negatively-charged species of the tripeptide Val-Leu-Ser at pH 7.0?

A: At pH = 7, the carboxyl terminal will have a full -1 charge. At pH = 7, we need to use…

Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.