Suppose you measure the absorbance of a yellow dye solution in a 1.00 cm cuvette (b=D1.00 cm). The absorbance of the solution at 427 nm is 0,41. If the molar extinction coefficient of vellow dye at 427 nm is 27400 M cm-1, what is the concentration of the solution in M? Type answer:

Substance P has an extinction coefficient of 72.22 mM-1cm-1 at 420nm. 2 μl of Substance P was added to 998 μl water and the Absorbance at 420nm was 0.820. Calculate the mM concentration of the original Substance P solution

SPECTROPHOTOMETRY Two groups in a BIO 120 lab extracted a blue-violet pigment from mayana plant which was to be used as a natural dye for Philippine textiles. To determine the concentration of the pigment isolated, each group made a standard curve using commercially available indigo dye in dimethylsulfoxide (DMSO). The following data were obtained by the two groups: Table 4. Absorbance readings of standard solutions of varying concentrations of indigo dye in DMSO. Pure DMSO 0.115 Standard solutions (ppm) Group 1 Data Group 2 Data 0.2 0.398 0.224 0.4 0.563 0.437 0.6 0.732 0.603 0.8 0.833 0.811 1.0 1.008 0.966 1. Show the constructed standard curves of the two groups with proper figure titles. 2. Which of the two groups generated a better standard curve? Justify your answer by providing the R? value for each data set. 3. The extracted pigment has an absorbance of 0.983. When a 0.2 mL-aliquot of the extract was diluted to 1.0 mL, the absorbance of the diluted solution was found to be…

A 2.42 x 10-5 M sample of compound X in a solvent has an absorbance of 0.313 at 528 nm in a 1.000 cm cuvel alone has an absorbance of 0.058. What is the molar absorptivity of compound X? 5 € = M-¹.cm=¹ A different sample of compound X in the same solvent has an absorbance of 0.485 at 528 nm when measured with the same cuvette. What is the concentration of this second sample? [X] = mol X L