Suppose that acid HA (dissociation constant Ka) is partitioned between aqueous phase 1 and organic phase 2 with partition coefficient Ko for HA. Assuming that A- is not soluble in the organic phase, show that the distribution coefficient is given by K (H*] D = = K•CHA %3D %3D [H*]+ K, Harris (9th) Equation: 23-7

Calculate the molar solubility of Zn(OH)2 in a solution of 0.0167 M K₂SO4.

The measurement of LDH in the serum can be used to diagnose whether tissue damage hasoccurred.Students were tasked with the development of an assay for LDH activity in human serum samplesfrom a control patient (healthy) and a patient who had suffered cardiac damage. Two samples wereprovided, labelled C and D (Control and Diseased serum).The students decided to follow the loss of absorbance of NADH in an appropriate assay buffercontaining pyruvate as the substrate. Q1 (a) By examining the absorbance spectra(in the image attached) of both NAD+ and NADH below, comment why this approach is suitable strategy. Q1 (b). What would be the optimum wavelength at which they should measure absorbance? Give a reason for your answer. The students conducted the assay for LDH activity using the two serum samples. Each cuvette (path length 1 cm) contained 3ml of a suitable assay buffer (including pyruvate as substrate). 20 microlitres of either serum was added to the cuvette and the absorbance values…

The answer is B. Please explain, thanks.