Standard Tubes Volume of 0.1 Hydroxamate standard Tubes Optical Density (OD) Concentration 0.061 0.078 0.095 1 0.1 0.2 0.4 0.002 3 4 0.004 0.125 0.008 Standard Curve of Optical Density (OD) vs Concentration of Hydroxamate 0.14 0.12 0.1 520 nm

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Chapter1: Chemical Foundations
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Standard Tubes
Optical Density
(OD)
Tubes
Volume of 0.1
Concentration
Hydroxamate
standard
1
0.061
0.1
0.078
0.002
3
0.2
0.095
0.004
4
0.4
0.125
0.008
Standard Curve of Optical Density (OD) vs Concentration of
Hydroxamate
0.14
0.12
0.1
0.08
ý = 7.9857x + 0.0618
R = 0.9988
0.06
0.04
0.02
0.001
0.002
0.003
0.004
0.005
0.006
0.007
0.008
0.009
Concentration of Hydroxamate
Figure 1: Graph of Standard Curve of Optical Density against the Concentration of
Hydroxamate.
Hydroxamate Assay Tubes
From Fig 1, the equation displayed was y = 7.9857x + 0.0618 and the R2-0.9988. From the
equation, y intercept was the optical density and x-intercept was the concentration of
hydroxamate.
Tubes
Optical Density at 520 nm
Concentration of
0.103
0.077
0.123
hydroxamate formed
0.0052
0.0019
0.0077
0.0092
6
7
0.135
0.114
0.0065
10
0.065
0.0004
Optical Density at 520 nm
Transcribed Image Text:Standard Tubes Optical Density (OD) Tubes Volume of 0.1 Concentration Hydroxamate standard 1 0.061 0.1 0.078 0.002 3 0.2 0.095 0.004 4 0.4 0.125 0.008 Standard Curve of Optical Density (OD) vs Concentration of Hydroxamate 0.14 0.12 0.1 0.08 ý = 7.9857x + 0.0618 R = 0.9988 0.06 0.04 0.02 0.001 0.002 0.003 0.004 0.005 0.006 0.007 0.008 0.009 Concentration of Hydroxamate Figure 1: Graph of Standard Curve of Optical Density against the Concentration of Hydroxamate. Hydroxamate Assay Tubes From Fig 1, the equation displayed was y = 7.9857x + 0.0618 and the R2-0.9988. From the equation, y intercept was the optical density and x-intercept was the concentration of hydroxamate. Tubes Optical Density at 520 nm Concentration of 0.103 0.077 0.123 hydroxamate formed 0.0052 0.0019 0.0077 0.0092 6 7 0.135 0.114 0.0065 10 0.065 0.0004 Optical Density at 520 nm
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