Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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Specific absorbance is the absorbance of a 1% solution of a given substance. The specific
absorbance can be calculated by:
A
1%
ст
C(i %
,
For the identification of a substance, 0.0482 g of substance has been weighed, which has been
dissolved in 50.00 ml of demineralized water. Thereafter, 50.0 µl of this solution has been
diluted to 50.00 ml. a UV-wise analysis (A = 249 nm) of this dilution gave an absorbance of 0.925.
a) Calculate the concentration of the diluted solution in% (w/v).
b) Calculate the specific absorbance of the substance.
c) Will it be possible to measure the specific absorbance directly on a 1% solution? Why / why not?
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Transcribed Image Text:Specific absorbance is the absorbance of a 1% solution of a given substance. The specific absorbance can be calculated by: A 1% ст C(i % , For the identification of a substance, 0.0482 g of substance has been weighed, which has been dissolved in 50.00 ml of demineralized water. Thereafter, 50.0 µl of this solution has been diluted to 50.00 ml. a UV-wise analysis (A = 249 nm) of this dilution gave an absorbance of 0.925. a) Calculate the concentration of the diluted solution in% (w/v). b) Calculate the specific absorbance of the substance. c) Will it be possible to measure the specific absorbance directly on a 1% solution? Why / why not?
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