Shown in the following table are several amino acid substitutions in the a and B chains of human hemoglobin. Use the genetic code table in Figure 12.7 to determine how many of them can occur as a result of a single nucleotide change. Substituted НЬ Туре НЬЈ Toronto ньЈ Оxford Normal Amino Acid Amino Acid Ala Asp (a-5) Gly Asp (a-15) Hb Gln lu (a-54) НЬ Bethesda Tyr His (B-145) Hb Sydney Val Ala (B-67) HbM Saskatoon His Tur (R-63)

Given the following diagram of how protein AWESOME1 binds to it's target DNA, describe the potential effects of each of the 5 mutations shown below. The wild-type sequence of a helix #1 is also shown in the blue box, and all the mutations are in helix #1 (see numbers for identifying particular residues). a helix #1 R(1)-V-I-L-Y-F-W-I-M-Y-F-S-H-Y-W-R(16) #1 Predict the consequence of the following mutations: 1) Arg(1) to Glu 2) Arg(1) to Ala 3) Phe(6) to lle 4) Trp(7) to Phe 5) Met(9) to Pro in