A3 Q21. A polypeptide is cleaved into peptides by treatment with trypsin and cyanogen bromide,and the peptides are purified and sequenced. The sequences of the peptides are shown below.(note: trypsin cleaves after lysine (K) and arginine (R); cyanogen bromide cleaves aftermethionine (M)). Work to show the sequence of peptide using either Ts or Cs. (6; 84)Trypsin peptideCyanogen bromide peptidesC1: TKDCVHSDT1: EGAAYHDFEPIDPRT2: GASMALIKC2:EGAAYHDFEPIDPRGASMT3: YLIACGPMTKC3: ALIKYLIACGPMT4: DCVHSD

Given: Trypsin peptides and Cyanogen bromide peptides has given.

Answered: s are purified and sequenced. The…

s are purified and sequenced. The sequences of th eaves after lysine (K) and arginine (R); cyanogen br .Work to show the sequence of peptide using eithe Cyanogen bromide C1: TKDCVHSD EPIDPR

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

Q21. A polypeptide is cleaved into peptides by treatment with trypsin and cyanogen bromide,
and the peptides are purified and sequenced. The sequences of the peptides are shown below.
(note: trypsin cleaves after lysine (K) and arginine (R); cyanogen bromide cleaves after
methionine (M)). Work to show the sequence of peptide using either Ts or Cs. (6; 84)
Trypsin peptide
Cyanogen bromide peptides
C1: TKDCVHSD
T1: EGAAYHDFEPIDPR
T2: GASMALIK
C2:
EGAAYHDFEPIDPRGASM
T3: YLIACGPMTK
C3: ALIKYLIACGPM
T4: DCVHSD

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