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- Consider the following prescription: Sol silver nitrate 0.5% 15mL Make solution isotonic for ophthalmic use. B. How many milligrams of potassium nitrate will need to be added to the prescription to make it isotonic? Report numerical value only rounded to a whole number. Need only handwritten solution only (not typed one).5. A tissue plasminogen activator (TPA) ophthalmic solution is prepared to con- tain 25 µg/100 μL. (a) Calculate the percentage concentration of TPA in the solution. (b) What volume of a solution containing TPA, 50 mg/50 mL, should be used to prepare each 100 μL of the opthalmic solution? 5. (a) 0.025% w/v TPA (b) 0.025 mL34. A dermatologic lotion contains 1.25 mL of liquefied phenol in 500 mL. Calculate the percentage (v/v) of liquefied phenol in the lotion. 34. 0.25% v/v liquefied phenol
- 2. For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. How much of the diluent must be added to tube 2 if 200uL Red cell suspension is added? Show pertinent solutions. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions.55. An ophthalmic solution is preserved with 0.0008% (w/v) of an antibacterial. Ex- press this concentration as a ratio strength. 55. 1:125,000 w/v8. R ACULAR (ketorolac tromethamine) Ophthalmic Solution 0.5% Disp. 5 mL Sig: One drop q.i.d. prn allergic conjunctivitis How many milligrams of the active con- stituent would be present in each drop of the ophthalmic solution if the dropper service delivers 20 drops per milliliter? (a) 0.25 mg (b) 25 mg (c) 0.025 mg (d) 1.25 mg 8. (a) 0.25 mg ketorolac tromethamine
- 3. Astelin nasal spray contains 0.1% azelastine hydrochloride and 400 µg/mL of benzalkonium chloride as a preservative. A container is capable of delivering 200 metered sprays of 0.137 mL each. The percent concentration of benzalkonium chloride in the preparation is ________ %, and this is equivalent to a ratio strength of 1:2500 w/v. True or False?2. Absorbance Data Table Absorbance Concentration 0 mg/dL = blank %3D 0.09 Oi17 0.28 25 mg/dL 50 mg/dL 75 mg/dL 100 mg/dL 035 Unknown solution 6.14 3. Attach a graph of your standard curve. Show the point for the unknown solution on your graph, and determine the concentration of the unknown. Enter this value below (include correct units). Concentration of the unknown solution1. What volume of sterile water for injection (diluent) must be added to reconstitue the powder? 2. How many mL of the reconstituted cefazolin is needed to prepare the final product?
- 6. The Worthington's manual reports two extinction coefficients for lysozyme. Corvert one into the other. What is the absorbance at 280 nm of a 0.1 mg/mL lysozyme solution? Use both coefficients to estimate the result7. Short answer. Professor Forni has hired vou as a research assistant and you need to make an X-gal staining solution for your sections containing 5mM potassium ferrocyanide, 5 mM potassium ferricyanide, 2 MM MgCl2, 0.1% Tween and 0.1% BCIP. You need 50 ml. As you are getting ready you discover that your colleague has used most of the BCIP and you only have 150 ul of the BCIP left. Is it enough? Show your calculations. 8. Short answer. Professor Scimemi has accepted vou as a Master's student and you are involved in a project that studies the cellular basis of neuropsychiatric diseases. For your electrophysiology recordings you have to make a recording solution containing (in mM): 119 NaCI, 2.5 KCI, 2.5 CaCl2, 1 MgCl2, 26.2 NaHCO3, 1 NaH¿PO4, 22 glucose. You need 800 ml of it. How much NaCl and how much KCl do you need to weigh out? (MW NaCI is 58.44 g/mol; MW of KCI is 74.55 g/mol) 9. You are working as a research technician for Pfizer and in your lab, one solution of hydrochloric…1. Explain the effectiveness of 70% alcohol as antiseptic compared to the action of 40% and 95% alcohol. 2. Which is more effective as an antiseptic: ethyl alcohol or isopropyl alcohol? Choose only one and explain why. 3. Which is more effective as a disinfectant: ethyl alcohol or isopropyl alcohol? Choose only one and explain why. 4. What is the difference between cleaning, sanitizing, and disinfecting?