Rank these steps:  The C-terminal fragment of the original peptide is released. A peptide side chain binds to the specificity pocket of chymotrypsin. The N-terminal fragment of the original peptide is released. Water binds to the active site of chymotrypsin.

What is the function of the signal sequence of a protein? How is itformed, and where is it located?

What is the binding between two amino acids called?

A sample of an unknown peptide was divided into two aliquots. One aliquot was treated with trypsin and the other was treated with cyanogen bromide. Given the following sequences (N-terminal to C-terminal) of the resulting fragments, deduce the sequence of the original peptide. Trypsin treatment Asn—Tyr—Asp—Met—Phe—Ala—Arg Asp—Trp—Asn—Arg Gln—Met—Tyr—Cys—Pro—Ile—Arg Gln—Cys Cyanogen bromide treatment Tyr—Cys—Pro—Ile—Arg—Asn—Tyr—Asp—Met   Asp—Trp—Asn—Arg—Gln—Met Phe—Ala—Arg—Gln—Cys