Question 8 In the following plasmid, if the region between the 2 sites for HgiEll were deleted, the resulting plasmid Clal (24) EcoRI (4359) Hindi (29) Sspl (4170) EcoRV (187) Apall (4033) BamHI (375) 0 4000 pBR322 4361bp Pstl (3611) HaiEll (3056)- 400 3200 3000 2800 Apall (2787) 3800 eta-lactamase gene 0096 2600 gri Anull 2400 2200 Hai Ell (2295) 200 400 600 tetracycline resistan 800 1800 1600 2000 rop Pvull (2066) 1000 1400 Sphi (566) 12.00 Sall (651)

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question 8
In the following plasmid, if the region between the 2 sites for HgiEll were deleted, the resulting plasmid
Clal (24)
EcoRI (4359) Hindi (29)
Sspl (4170)
EcoRV (187)
Apall (4033)
BamHI (375)
0
4000
pBR322
4361bp
Pstl (3611)
HaiEll (3056)-
400
3200
3000 2800
Apall (2787)
3800
eta-lactamase gene
0096
2600
2400 2200
HalEll (2295)
(A) would be about 2.6 kbp in length
B would be useless as it would lack an origin of replication
C would work well for cloning
(D) would confer resistance to ampicillin but not tetracycline
gri
Afull
200
400
600
tetracycline resistan
800
1800 1600
2000
rop
Pvull (2066)
1000
1400
Sphi (566)
12.00
Sall (651)
Transcribed Image Text:Question 8 In the following plasmid, if the region between the 2 sites for HgiEll were deleted, the resulting plasmid Clal (24) EcoRI (4359) Hindi (29) Sspl (4170) EcoRV (187) Apall (4033) BamHI (375) 0 4000 pBR322 4361bp Pstl (3611) HaiEll (3056)- 400 3200 3000 2800 Apall (2787) 3800 eta-lactamase gene 0096 2600 2400 2200 HalEll (2295) (A) would be about 2.6 kbp in length B would be useless as it would lack an origin of replication C would work well for cloning (D) would confer resistance to ampicillin but not tetracycline gri Afull 200 400 600 tetracycline resistan 800 1800 1600 2000 rop Pvull (2066) 1000 1400 Sphi (566) 12.00 Sall (651)
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