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- Quèstion 34 Enzyme that joins the DNA fragments O Recombinant DNA Technology O Restriction enzymes O Ligase O Palindromic sequencesQUESTION 17 OL NEere interested in generating a PCR amplicon including the bracketed sequence below. Which of the following sequences would be canen hybridizing (annealing) with the target AND would also serve to generate a copy of the bracketed region of interest? 5'-AATCGT[AGCAGCAGCAGTGGCT]A AGCT-3 3' -TTAGCA[TC GTC GTC GTC ACC G A] TTCG A - 5' 3-TTAGC-S S-AATCG-3 OSAAGCT-3 5-AGCTT-3 5-GCTAA-3 5-TCGAA-3 QUESTION 18 Vhich of the following is/are true regarding the enzvme PRIMASE? Save and Submit to save and submit. Click Save All Answers to save all answers.QUESTION 23 Michelle has a clone of the DNA of a newly discovered virus. She wants to identify which specific cells of an organ are infected by the virus. What method would be the most useful to answer this question? O restriction fragment analysis O microinjection of the gene fragment O Sanger sequencing of the clone O FISH (fluorescent in situ hybridization) O real time PCR QUESTION 24 Mutagens are useful in biotechnology research for Click Save and Submit to save and submit. Click Save All Answers to save all answers. Save All Answe
- QUESTION 19 Match the major enzyme involved in the techniques/steps we discussed in class v PCR A. Reverse Transcriptase v CDNA synthesis B. any enzyme that can cleave a chromogenic substrate v Cutting a plasmid for cloning C. Cas9 v "Gluing" an insert into a plasmid D. DNA polymerase v ELISA E. Restriction enzymes v Knocking out a gene at a specific targeted sequence F. LigaseQuestion 5 Review Figure 21.6 types of DNA sequences in the human genome. The lowest percentage of human genome O repetitive DNA related to transposable elements and related sequences (L1 sequences and Alu elements) O repetitive DNA unrelated to transposable elements (simple sequence and large-segment duplications O unique noncoding DNA O introns O exons O regulatory sequencesQuestion 5 Review DNA sequencing and cloning tools. Which of these is not used to make a recombinant DNA? O restriction enzymes to create sticky ends of a plasmid O fragment from a different DNA cut by the same restriction enzyme O DNA ligase seals the recombinant DNA O denaturation
- QUESTION 7 After you graduate, you are working at a laboratory that identifies a novel protein called Leprechaun. To determine in which organelles Leprechaun is found, you decide to express the Leprechaun gene in HeLa cells using a plasmid. Since you have generated an antibody that recognizes an epitope within the Leprechaun protein, which technique will you use to identify its localization within the HeLa cells? OA. SDS-PAGE O B. Immunocytochemistry O C. Western blot OD. Transmission electron microscopy (TEM) OE. Immunohistochemistry QUESTION 8 Which of the following statements regarding electron microscopy is TRUE? OA. White light is utilized to illuminate the sample for imaging in all types of electron microscopy. OB. Electrons pass through the object being examined in transmission electron microscopy (TEM). OC. In scanning electron microscopy (SEM) electrons, electrons bounce off the surface of the sample. OD. Electron microscopy can be used to view structures as small as 2 nm in…Question 3. Biochemistry techniques have been used for the analysis on biomolecules such as enzymes, DNA, antibodies and other molecules in biomedical engineering. a) Polymerase chain reaction (PCR) is an artificial method to amplify DNA segment into thousand copies of that DNA. i. Elaborate the three steps in PCR which based on temperature manipulation in controlling the replication process. Distinguish the uniqeness of Reverse Transcriptase PCR (RT-PCR) technique as ii. compared to the common PCR. iii. Relate the application of RT-PCR and Rapid Diagnostic Test for biomarker detection with the typical time course of COVID-19 infection.QUESTION 3 You are provided with plasmid DNA at 200ng/uL, 10X restriction digestion buffer, restriction enzymes (1 unit/ul) and water. You want to digest 1.5 ug of plasmid DNA with 1 unit (U) of enzyme in 1X buffer in a total volume of 80 ul. Complete the table below: Solution Stock solution Working solution Volume needed restriction digestion buffer 10X 1X DNA 200 ng/ul 1.5 με enzymes 1 unit/ul 1 unit Water Total 80 μL
- Question 35 Enzymes used in Recombinant DNA Technology O Recombinant DNA Technology O Restriction enzymes O Restriction Enzymes and Ligase O Palindromic sequencesQuestion 13 The reason Toq polymerase is specifically desirable for use in PCR is that it does not (itself) denature at the high temperatures necessary to denature DNA. O True O FalseQuestion 32 Enzyme that combines the 2 DNA fragments from different organisms Recombinant DNA Technology Restriction enzymes O Ligase O Palindromic sequences