Question 20. Typically cloning of any DNA fragment involves the following tasks: Put these tasks in the correct order. a. Joining the fragment with the vector. b. Preparing DNA fragments to be cloned. c. Preparing a vector for cloning. d. Selecting for bacterial cells containing the plasmid with the insert. e. Introducing the hybrid vector into bacteria. f. Isolation of plasmid with insert from bacterial cells.

Question 20. Typically cloning of any DNA fragment involves the following tasks: Put these tasks in the correct order. a. Joining the fragment with the vector. b. Preparing DNA fragments to be cloned. c. Preparing a vector for cloning. d. Selecting for bacterial cells containing the plasmid with the insert. e. Introducing the hybrid vector into bacteria. f. Isolation of plasmid with insert from bacterial cells.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 11QP: Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA...

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Question 1. Restriction endonucleases can be isolated from a number of bacteria. In bacteria restriction endonucleases. a-restrict chromosomal DNA that is heavily mutated b-restrict chromosomal DNA with significant regions that have been Deleted. c-restrict the DNA of invading bacteriophages. d- all of the above Question 2. In a PCR reaction the step in which DNA polymerase replicates the DNA is referred to as a- denaturation b- annealing C- extension d- initiation
5) Answers to the following questions. A. You are given a tube containing 275 ng of purified PCR product (DNA) that is 1262 bp long. How many picomoles of PCR product are in the tube? The average weight of a deoxynucleotide monophosphate is 328 g/mol. B. You digest a 5 kb plasmid with two enzymes that yield fragments of 4 kb and 1 kb, and resolve the digest on the gel. What would you predict to see in terms of the intensities of the bands?
Question 2. Retroviruses are used in gene therapy. The goal of gene therapy is to insert in the patient genome a copy of a functional gene that is defective in the patient. Since Retroviruses integrates their genome into the host genome they are ideal gene therapy delivery systems. What would be a potential risk of this type of treatment? The individual treated could be more susceptible to infection by other retroviruses Insertion of the retroviral genome into the host genome can cause dangerous mutations. There are not recognized risks with this gene therapy approach. Genes from the host can be inserted into the retroviruses and laterally moved to other cells.
Question:- Viral vectors are not the only method being studied for gene therapy purposes. Which of the following is a nonviral delivery method for gene therapy? a.gene pills b.DNA bound to the surface of liposomes c.electrically stimulating cells to take up DNA d.DNA covered with protein
Question 7 Referring to the plasmid below, if a recombinant plasmid were obtained by inserting DNA into the EcoRv site, and the protein corresponding to the recombinant gene were expressed, which of the following statements would be false? Aval Sall A The plasmid will be resistant to tetracycline. B) The protein is not likely to be biologically active without some further treatment. C) The plasmid will be resistant to ampicillin. (D) The plasmid will not be able to replicate autonomously. Pul- Poul- ampr III EcoRI EcoRV BamHI pBR322 (4363 bases) -Poudl lett
QUESTION 5: Should PCR primers be complementary to each other? Explain the reasoning for your answer.
Please answer both Part a. The plasmid you used as a template in your positive control for your PCR is 3300 bp in size. Why is the PCR product only 650 bp long? b. You using PCR to check for the presence of the GFP gene, however the thermocyder (machine that through temperatures) has been misprogrammed and tge annealing temperature is set to 43c (low for your primers) Explain what you would expect to see on your gel and why?
Complete the following problems. Restriction enzymes (REs), which cut D NA at specific sequences, are classic tools in molecular biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences by analyzing the fragments generated upon restriction digest, as in the example shown in Figure 1. Your task is to study the circular plasmid, pMBBS, through restriction digests. You subjected the pMBBS plasmid to complete digestion by different combinations of three REs (EcoRI, BamHI, and Xhol), and analyzed the results on an agarose gel, shown below. Using the data you can glean from this gel, answer the questions that follow. EcoRI BamHI Xhol *The same total amount of DNA was loaded in each lane. 1. What is the total size of the pMBBS plasmid in bp? Answer: bp + DNA size ladder 2. How many cut sites on the PMBBS plasmid does each RE have? EcoRI: BamHI: Xhol: 3000 bp 2500 bp -2000 bp -1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp…
QUESTION 8 What is the function of the amp' gene in a vector? A. It is a restriction enzyme recognition site.. B. It is a selectable marker. O C. It is the origin of replication. O D. It makes the enzyme ß-galactosidase if it is intact, indicating vectors without inserts. O E. It is required for packing into viral shells
Question 6 Plasmids that generate blunt ends after restriction enzyme digestion are the ones most effective for recombinant DNA technology. A True B) False
In gene cloning, a vector is required to transform the gene of interest into host cell. State the THREE (3) important regions of the plasmid. Elaborate your answer.
QUESTION 5 Imagine that you have cloned the gene encoding the SARS-CoV-2 spike protein to a plasmid vector. The next thing is to put the plasmid into a host. Describe how you can transform the plasmid containing the gene encoding the SARS-CoV-2 spike protein.