Question 20. Typically cloning of any DNA fragment involves the following tasks: Put these tasks in the correct order. a. Joining the fragment with the vector. b. Preparing DNA fragments to be cloned. c. Preparing a vector for cloning. d. Selecting for bacterial cells containing the plasmid with the insert. e. Introducing the hybrid vector into bacteria. f. Isolation of plasmid with insert from bacterial cells.

Identify the following labeled structures.

What should you do if you feel resistance while inserting the needle into the injection port during GC? Remove the needle and retry the injection Keep going, there should be lots of resistance in this injection Get a new needle as this one is broken Restart the GC machine with the help of your TA

1. Which of the following makes it especially difficult for veterinarians to determine if an opioid is truly indicated? A. Opioids used in veterinary medicine are not used in human medicine B. Our patients can’t talk C. Our patients always require larger doses than humans D. All of the above   2. Which of the following is/are potential problems with PDMPs in veterinary medicine? A. Determining how to report the data B. Potential HIPPA violations when reviewing the system seeing the client’s medical information C. How to tie pets to the people that we are trying to monitor D. All of the above   3. Which schedule of controlled substance has no accepted medical use? A. CI B. CII C. CIII D. CIV   4. Which state specific regulatory agencies do you need to be familiar with to know how they regulate controlled substances at the state level? A. Local DEA Field Office B. State Board of Pharmacy C. State Veterinary Board D. All of the…