Question 14 Select all of the following that will slow down enzyme activity but not denature the enzyme. adding significant amounts of acid or base lowering temperature heating to high temperatures noncompetitive inhibition competitive inhibition

QUESTION 39 The metabolic reaction pathway that is responsible for the generation of the most reducing equivalents is :

QUESTION 1 You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase), and your goal is to obtain purified ẞ-gal. Some characteristics of these proteins are shown in the table below Protein Alcohol dehydrogenase Carbonic anhydrase Insulin B chain Phosphorylase B Glutamic dehydrogenase B-galactosidase Concentration of ammonium sulfate (AS) required for precipitation Molecular Weight (kDa) Isoelectric point (pl) 45% 38 3.7 80% 65% 20% 30% 45% 28 4.8 4 5.3 98 6.8 . 49 9.5 115 5.3 You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropriate concentration of AS to your CL sample, incubate overnight at 4°C, then centrifuge to generate a supernatant (AS-S) and pellet (AS-P). What concentration of AS will you use to precipitate ẞ-galactosidase? 20% 30% 45% 65% O 80%

All the Bold answers are wrong please explain why it is wrong and give me the correct answer. Thanks, in advanced Which of the following is true about enzymes? Enzymes are equally effective across broad ranges of temperature and Ph. Enzymes lower the overall free energy of a reaction to make it spontaneous. Enzyme activity is generally unregulated. Enzymes recognize many substrates with equal specificity. The insulin receptor catalyzes the phosphorylation of several substrates and is therefore classified as a Transferase Phosphate Kinase Lyase Oxidoreductase  3)Which of the following reaction parameters can enzymes optimize to increase reaction rate? The proximity(=closeness) of the reacting groups. The rotational motions of the substrates and catalytic groups. The orientations of the substrates and catalytic groups. The achieve energy needed to achieve the transition state.   The catalytic mechanism of RNA relies upon general acid-base catalysis involving the amino acid…