QUESTION 1 You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase), and your goal is to obtain purified ẞ-gal. Some characteristics of these proteins are shown in the table below: Protein Alcohol dehydrogenase Carbonic anhydrase Insulin B chain Phosphorylase B Glutamic dehydrogenase B-galactosidase Concentration of ammonium sulfate (AS) required for precipitation Molecular Weight (kDa) Isoelectric point (pl) 45% 38 3.7 80% 28 4.8 65% 4 5.3 20% 98 6.8 30% 49 45% 115 9.5 5.3 You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropriate concentration of AS to your CL sample, incubate overnight at 4°C, then centrifuge to generate a supernatant (AS-S) and pellet (AS-P). What concentration of AS will you use to precipitate Alcohol dehydrogenase? ○ 20% 30% 45% 65% 80%

Which statement about the glycoprotein shown below is true? OH CH₂OH OH HN HN C - C=0 CH3 H₂ Protein Xo CH N-H Protein It contains a deoxyribose sugar that could be metabolized by human enzymes. The monosaccharide is attached to the protein via nitrogen in a residue's side chain. It contains a ribose sugar that could not be metabolized by human enzymes. O The monosaccharide is attached to the protein via nitrogen in a residue's a-amine.

23. In which location would you find the molecule glucose? Explain your reasoning. HO HO CH₂OH -OH (8) OH1 8-D-glucopyranose (chair conformation) 24. In which location would you find the molecule vitamin K? Explain your reasoning. двалла Vitamin K₁

14. In the figures shown below, identify the glycosidic bond by placing an "x" on it. Label the type of glycosidic bond using the numbers of the carbon and alpha/beta symbol CH₂OH 1 вна HỎ OH OH CH₂OH bww HO OH