Question 1: Imagine you are a scientist working in an immunology lab and you are studying a new virus that is similar to Influenza virus that can activate CTL immune response. Your job is to study the epitopes in the viral spike protein and understand which epitope can generate a potent immune response. You would like to then use this data to formulate a vaccine for the virus. Answer the following questions based on your knowledge of CTL immune response, infectious diseases and vaccination. a) Design an experiment that you can use to study the various epitope peptides of the antigen on activating a CTL immune response. (Hint: Pulsing) b) Create a hypothetical data table to show which peptide resulted in effective lysis by CTL cells (Hint: Refer to the end of Chapter 12). c) Based on the data you have created, which peptide effectively resulted in lysis. d) How would you go about designing a vaccine using the epitope peptides you have designed? Talk about the methods, trials and safety considerations while designing the vaccine. e) What are the steps to consider before the vaccine is approved for use in humans?

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Can Alex be Frank’s father?  Why or why not?

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