Q5) What genus of bacteria is most likely to produce antibiotics? Q6) How could you tell if antibiotics are actually produced? Describe for me what was done in the lab to confirm this.
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Q5) What genus of bacteria is most likely to produce antibiotics?
Q6) How could you tell if antibiotics are actually produced? Describe for me what was done in the lab to confirm this.
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- 1) What is NDM-1? How can NDM-1 spread to different types of bacteria? 2) Why are Gram negative bacteria so much harder to treat with antibiotics? 3) What is KPC? Where does it live?A suspected pathogen is observed from the blood of a diseased mouse. An investigation of the other mice in the area indicates that it is not present in healthy mice. With Koch's postulates in mind, what is the next step to determine whether or not this suspected pathogen is the cause of disease in the mouse? a) Grow the suspected pathogen in a pure culture Ob) Re-isolate the suspected pathogen and show that it is the same as the original pathogen c) Inoculate a healthy mouse with the suspected pathogen O d) Determine whether the organism in the pure culture is the same one as in the original sampleWhich of the following is not a method used to introduce foreign DNA into a bacterial cell? a) biolistic gun b) calcium chloride treatment followed by heat shock c) infection with a specific phage d) electroporation
- 1. Which of the following bacterial species is not naturally competent? A) Bacillus subtilis В) Наеторhilus influenzae C) Escherichia coli D) Streptococcus рпеuтoniae 2. Bacterial species which are not naturally competent can be made competent in the laboratory by using a combination of metal ions and temperature changes. A) true B) false 3. If 10 ng of plasmid DNA yielded 10,000 transformants per milliliter (mL), the transformation efficiency is: A) 105 В) 105 C) 107 D) 105 4. Under optimal conditions, all the bacterial cells in a sample can be transformed. A) true B) false 5. The plasmid called pGal, carries a gene encoding the enzyme beta-galactosidase. What is the natural function of this enzyme? A) It protects cells from a specific type of antibiotic. B) It hydrolyzes the disaccharide lactose. C) It is involved in plasmid replication. D) It is necessary for transformation. 6. The pGal plasmid also carries a gene designated as amp". What is the function of this gene? A) It protects…Which of the following is TRUE when one assay bacteriophage titers? You should: a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate b) directly add the phage dilution onto the surface of an agar plate c) add tryptic soy broth to the phage dilution and incubate overnight d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar plate31) To establish a link between a specific bacterium and a skin disease, researchers have shown that the bacterium was present in sick persons but not in healthy individuals. They isolated the bacterium in a pure culture and demonstrated that experimental healthy animals injected with this culture became sick. What other experiment do researchers need to perform to be absolutely sure that the bacterium is responsible for the disease? A) Demonstrate that the bacterium belongs to the pathogenic lineage B) Demonstrate that the bacterium is not able to live outside of humans or animals C) Isolate bacterium from an infected sick animal and demonstrate that it is the same bacterium as the one used for infection D) Isolate bacterium from an infected sick animal and demonstrate that it is susceptible to broad spectrum antibiotics.
- Attached below is the study about the effects of handwashing and the effectiveness of soap against bacteria. What conclusions can be generated from this result? A C before washing hands 10 second hand wash B D hot water only rinse 30 second hand wash Figure 8: Agar plates with bacteria A) before washing hands B) after rinsing hands in hot water C) After washing hands with soap for 10 seconds and D) after washing hands for 30 seconds. Images show that the number of bacteria on the plate are not greatly reduced after a hot water rinse or 10 second handwash but bacterial levels are reduced after a proper hand wash for 30 seconds.7) Identify the most correct choice: a) The time required for a bacterial population to double is called generation time Ob) Nitrogen is required by the organisms mainly to produce ATP. c) Agar is a common food source for the bacteria. d) A colony is usually a mixture of various bacterial organisms. e) a and ca) What is morphology? b) Describe the common morphological characteristics of bacteria that are often used in microbiological studies. c) Explain the chemical and physical requirements needed for bacterial growth d) Explain the importance of using growth medium and devise a STEP-BY-STEP plan to grow bacterial cell cultures in the laboratory.
- When do we make the determination that a bacterium is Gram positive? A) Safranin washes the crystal violet die out of the cell wall. B) Crystal violet die is not able to absorb into the cell wall. C) Alcohol does not wash the crystal violet die out of the cell wall. D) Crystal violet turns the bacterium a yellow color.13) A certain bacterial colony originated from the division of a single bacterial cell. Each cell in this colony will most likely A) express adaptations unlike those of the other cells B) synthesize the same proteins and enzymes C) have a resistance to different antibiotics D) replicate different numbers of genes10) If the bacterium Staphylococcus aureus experiences a cost for maintaining one or more antibiotic- resistance genes, what would happen in environments that lack antibiotics? A) These genes would be maintained in case the antibiotics appear. B) These bacteria would be outcompeted and replaced by bacteria that have lost these genes. C) These bacteria would try to make the cost worthwhile by locating and migrating to microenvironments where traces of antibiotics are present. D) The number of genes conveying antibiotic resistance would increase in these bacteria. 11) When nonrandom mating occurs in a population so that individuals prefer to mate with similar individuals, allele frequencies should A) remain the same, but homozygotes will be overrepresented in the population. B) remain the same, but heterozygotes will be overrepresented in the population. C) change and heterozygotes will be overrepresented in the population. D) change and homozygotes will be overrepresented in the…