Q1) Why are there 2 different ‘stops’ on the micropipette? Q2) There’s many mistakes you can make micropipetting. Describe 3 of them Q4) What does a P100 pipette mean? P20?
Q1) Why are there 2 different ‘stops’ on the micropipette? Q2) There’s many mistakes you can make micropipetting. Describe 3 of them Q4) What does a P100 pipette mean? P20?
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter11: Instrumentation In Respiration
Section: Chapter Questions
Problem 3P
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Q6) Why would a sample be over 100%? Do you think 110% accurate is the same as 90% accurate?
Q1) Why are there 2 different ‘stops’ on the micropipette?
Q2) There’s many mistakes you can make micropipetting. Describe 3 of them
Q4) What does a P100 pipette mean? P20?
![Often pipettes need to be tested for accuracy
You would use a digital balance you to determine how accurate your pipet is and how
accurate you are at pipetting. The rule of thumb is that 1 g =1 ml. If you pipet 540 ul of water
onto a digital scale it should measure 0.54 g because 1 ml = 1,000 ul; therefore 540 ul =
0.54 ml = 0.54 g.
Q5) How accurate are these pipettes? (the first one is done for you)
Weight
(g)
Set volume
% Accuracy
Micropipette
(ul)
= [(weight x 1,000) volume] x 100
P20
15.0
0.012
[(0.012 x 1,000) + 15] x 100 = 80%
P200
32.0
0.033
P1000
547.0
0.525](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa925c6f0-ebb9-4852-9e39-723f42286d4d%2F538b3c6d-a6c7-4965-805f-2ec9bbf41e95%2Fz3h5eye_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Often pipettes need to be tested for accuracy
You would use a digital balance you to determine how accurate your pipet is and how
accurate you are at pipetting. The rule of thumb is that 1 g =1 ml. If you pipet 540 ul of water
onto a digital scale it should measure 0.54 g because 1 ml = 1,000 ul; therefore 540 ul =
0.54 ml = 0.54 g.
Q5) How accurate are these pipettes? (the first one is done for you)
Weight
(g)
Set volume
% Accuracy
Micropipette
(ul)
= [(weight x 1,000) volume] x 100
P20
15.0
0.012
[(0.012 x 1,000) + 15] x 100 = 80%
P200
32.0
0.033
P1000
547.0
0.525
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