Provide a brief explanation of one specific application on High Performance Liquid Chromatography (HPLC). Incorporate the specific application name (e.g., Detection and Quantification of Mycotoxins), provide aconcise overview of sample preparation methods, outline instrumental parameters and conditions utilized, and summarize the outcomes and findings achieved through this analytical approach.

Researchers used gas chromatography to determine the % v/v methyl salicylate in rubbing alcohol [Van Atta, R. E.; Van Atta, R. L. J. Chem. Educ. 1980, 57, 230-231]. A set of standard additions was prepared by transferring 20.00 mL of rubbing alcohol to separate 25 - mL volumetric flasks and pipeting 0.00 mL, 0.20 mL, and 0.50 mL of methyl salicylate to the flasks. All three flasks were diluted to volume using isopropanol. Analysis of the three samples gave peak heights for methyl salicylate of 57.00 mm, 88.5 mm, and 132.5 mm, respectively. Determine the % v/v methyl salicylate in the rubbing alcohol.

A researcher was attempting to quantify the amount of dichlorodiphenyltrichloroethane (DDT) in spinach using gas chromatography mass spectrometry (GCMS). First attempts using external calibration proved difficult, as reproducibility and external standard agreement were poor. To overcome these problems, the researcher used the internal standard calibration method with chloroform as the internal standard. To begin, the researcher analyzed a sample containing 5.67 mg/L DDT standard and 1.84 mg/L chloroform as the internal standard, which produced peak areas of 4035 and 10177 for the DDT and chloroform, respectively. After establishing the response factor of the instrument, the researcher collected 11.53 g of spinach, homogenized the sample, and extracted the DDT using an established method, producing a 3.70 mL solution containing an unknown amount of extracted DDT. The researcher then prepared a sample for analysis that contained 1.00 mL of the unknown DDT solution and 2.00 mL of 10.25…

(4) Below are depictions of a student's TLC plates in which fractions 1 - 10 from their column are spotted. Lane 1 2 3 4 5 67 8 9 10 (a) Which fractions contain pure fluorenone? Which contain pure ferrocene? (b) Which lanes, if any, represent mixed fractions? (c) Would the student incorporate lanes 6 and 7 into an Erlenmeyer flask of a combined fraction? Explain with 10 words or less.

A researcher was attempting to quantify the amount of dichlorodiphenyltrichloroethane (DDT) in spinach using gas chromatography mass spectrometry (GCMS). First attempts using external calibration proved difficult, as reproducibility and external standard agreement were poor. To overcome these problems, the researcher used the internal standard calibration method with chloroform as the internal standard. To begin, the researcher analyzed a sample containing 7.13 mg/L DDT standard and 1.28 mg/L chloroform as the internal standard, which produced peak areas of 4191 and 10585 for the DDT and chloroform, respectively. Determine the response factor (F). F =

Below are responses generated using the isolated guinea-pig ileum preparation to determine a pA2 value for the cholinergic antagonist homatropine.Responses are expressed in millimetres +/- SDM (n = 4).Plot two data graph on a sheet or excell and then determine by Schild analysis the pA2 value and Schild slope. show calculationsWhat conclusions can you draw; does the experiment/data fulfil the criteria for a valid Schild analysis?

(i) Calculate the capacity factors (k') for both compounds A and B and the selectivity factor (a) between them, commenting on the values. (ii) Calculate the resolution between A and B and comment on it. (iii) Using the peak for A, calculate the number of theoretical plates for the GC column and comment on the quality of the column.

The concentration of purified OXA-M290 is tested with a BCA assay. Serial dilutions of a bovine serum albumin (BSA) stock solution are prepared, then pipetted into a 96-well plate; each dilution of the BSA standard is tested in triplicate. Then, bicinchoninic acid and Cu2+ ions are added to all of the wells of the plate. After incubating the plate for 1 hour, a microplate reader is used to measure the absorbance of all of the wells in the plate at 560 nm. This generates the following data:  BSA conc. (μg/mL), Replicate 1 Absorbance, Replicate 2 Absorbance, Replicate 3 Absorbance  40, 1.360, 1.403, 1.481  20, 0.750, 0.745, 0.810  10, 0.380, 0.344, 0.398  5, 0.198, 0.160, 0.183  2.5, 0.090, 0.100, 0.085  1.25, 0.038, 0.043, 0.051  0.625, 0.024, 0.028, 0.019     Prepare a calibration curve using these data. You can use Excel, R, SPSS or an equivalent graphing software.  In this graph, plot absorbance (y-axis) against the concentration of the protein standard (x-axis). Calculate and plot…

Classify the stationary phases based on whether they are commonly used in reversed-phase liquid chromatography (RPLC) or normal-phase liquid chromatography (NPLC). RPLC NPLC Answer Bank octadecyl (–(CH,)17CH,) amino (-(CH,)3NH2) cyano (-(CH,),C=N) pentafluorophenyl (-(CH,);C,F;) diol (-(CH,),ОСH, СНIОH)СH,ОН) octyl (-(CH,),CH;) phenyl (-(CH, ),C,H;)

The following statements are true for any chromatographic analysis except: The analyte component interacting most strongly with the stationary phase elute faster than those with weaker interactions. On the other hand, those with least interaction with the mobile phase elute the slowest. Interactions between mobile phase-analyte - stationary phase can be both chemical and physical in nature. Chromatography is a separation technique. Electromagnetic radiation source for XPS. Gamma ray X-ray UV-Visible Infra red Radiowave The part of SEM that is responsible for the production of electrons. * Electron guns Condenser lens Secondary detector Electron beam