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Protein Concentration Prelab - Saved X Amelia Rennie AutoSave Search Off Design Mailings Review Share File References View Help Home Insert Draw Layout Comments Find Cut 11 A А А Аa v Cambria AаBЬСcDc AaBЬСcDc AaBbC AаBbСcL A ав Replace Copy Paste Dictate T Normal T No Spac... Heading 1 Heading 2 Title В IUvab х, х* А Select Format Painter Clipboard Paragraph Styles Editing Voice Font Also, convert the desired mg/mL concentration values to their equivalent in M (micromolar; 10-6 М). С1 (initial V1 (volume C2 (desired final conc of dilute BSA) V2 (volume Vol of Concentration of dilute soln in uM: of stock BSA of dilute concentration water to add solutions) of stock to add to tube) solution) 10 mg/mL 1.0 mg/mL 1.0 mL 10 mL 9.0 mL 0.8 mg/mL 10 mg/mL 0.8 mL 10 mL 9.2 mL 10 mg/mL 0.6 mg/mL 0.6 mL 10 mL 9.4 mL 0.4 mg/mL 10 mg/mL 9.6 mL 0.4 mL 10 mL 10 mg/mL 0.2 mg/mL 9.8 mL 0.2 mL 10 mL Calculation Examples: 1) V1 (10 mg/mL) V1 (0.8 mg/mL) (10 mL) 2)Vol. of water to add (V2 - V1) V1 = 0.8 mL LFocus 650 words Page 2 of 3 120% 12:22 PM PType here to search 12 1 10/17/2019

Protein Concentration Prelab - Saved X Amelia Rennie AutoSave Search Off Design Mailings Review Share File References View Help Home Insert Draw Layout Comments Find Cut 11 A А А Аa v Cambria AаBЬСcDc AaBЬСcDc AaBbC AаBbСcL A ав Replace Copy Paste Dictate T Normal T No Spac... Heading 1 Heading 2 Title В IUvab х, х* А Select Format Painter Clipboard Paragraph Styles Editing Voice Font Also, convert the desired mg/mL concentration values to their equivalent in M (micromolar; 10-6 М). С1 (initial V1 (volume C2 (desired final conc of dilute BSA) V2 (volume Vol of Concentration of dilute soln in uM: of stock BSA of dilute concentration water to add solutions) of stock to add to tube) solution) 10 mg/mL 1.0 mg/mL 1.0 mL 10 mL 9.0 mL 0.8 mg/mL 10 mg/mL 0.8 mL 10 mL 9.2 mL 10 mg/mL 0.6 mg/mL 0.6 mL 10 mL 9.4 mL 0.4 mg/mL 10 mg/mL 9.6 mL 0.4 mL 10 mL 10 mg/mL 0.2 mg/mL 9.8 mL 0.2 mL 10 mL Calculation Examples: 1) V1 (10 mg/mL) V1 (0.8 mg/mL) (10 mL) 2)Vol. of water to add (V2 - V1) V1 = 0.8 mL LFocus 650 words Page 2 of 3 120% 12:22 PM PType here to search 12 1 10/17/2019

Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
8th Edition
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:William L. Masterton, Cecile N. Hurley
Chapter23: Organic Polymers, Natural And Synthetic
Section: Chapter Questions
Problem 29QAP: For alanine, Ka1=5.1105,Ka2=1.81010 . Calculate the ratios [Z]/[C+] and [Z]/[A+] at pH (a) 2.00. (b)...
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BSA for concentration measurements is often prepared as a 10 mg/mL stock solution.  Suppose you wish to prepare a series of 10 mL solutions of BSA at 1.0, 0.8, 0.6, 0.4, and 0.2 mg/mL.  Using the dilution equation C1 × V1 = C2 × V2, you plan to do this by transferring a calculated volume (V1) of the 10 mg/mL (C1) into a clean test tube, then adding water in sufficient volume to produce 10 mL (V2) at the desired concentration (C2).  Complete the table below, showing the volume of stock 10 mg/mL solution you need to add to each tube, and the volume of water you need to add to each.  Also, convert the desired mg/mL concentration values to their equivalent in μM (micromolar; 10-6 M).

Protein Concentration Prelab - Saved X Amelia Rennie AutoSave Search Off Design Mailings Review Share File References View Help Home Insert Draw Layout Comments Find Cut 11 A А А Аa v Cambria AаBЬСcDc AaBЬСcDc AaBbC AаBbСcL A ав Replace Copy Paste Dictate T Normal T No Spac... Heading 1 Heading 2 Title В IUvab х, х* А Select Format Painter Clipboard Paragraph Styles Editing Voice Font Also, convert the desired mg/mL concentration values to their equivalent in M (micromolar; 10-6 М). С1 (initial V1 (volume C2 (desired final conc of dilute BSA) V2 (volume Vol of Concentration of dilute soln in uM: of stock BSA of dilute concentration water to add solutions) of stock to add to tube) solution) 10 mg/mL 1.0 mg/mL 1.0 mL 10 mL 9.0 mL 0.8 mg/mL 10 mg/mL 0.8 mL 10 mL 9.2 mL 10 mg/mL 0.6 mg/mL 0.6 mL 10 mL 9.4 mL 0.4 mg/mL 10 mg/mL 9.6 mL 0.4 mL 10 mL 10 mg/mL 0.2 mg/mL 9.8 mL 0.2 mL 10 mL Calculation Examples: 1) V1 (10 mg/mL) V1 (0.8 mg/mL) (10 mL) 2)Vol. of water to add (V2 - V1) V1 = 0.8 mL LFocus 650 words Page 2 of 3 120% 12:22 PM PType here to search 12 1 10/17/2019
Transcribed Image Text:Protein Concentration Prelab - Saved X Amelia Rennie AutoSave Search Off Design Mailings Review Share File References View Help Home Insert Draw Layout Comments Find Cut 11 A А А Аa v Cambria AаBЬСcDc AaBЬСcDc AaBbC AаBbСcL A ав Replace Copy Paste Dictate T Normal T No Spac... Heading 1 Heading 2 Title В IUvab х, х* А Select Format Painter Clipboard Paragraph Styles Editing Voice Font Also, convert the desired mg/mL concentration values to their equivalent in M (micromolar; 10-6 М). С1 (initial V1 (volume C2 (desired final conc of dilute BSA) V2 (volume Vol of Concentration of dilute soln in uM: of stock BSA of dilute concentration water to add solutions) of stock to add to tube) solution) 10 mg/mL 1.0 mg/mL 1.0 mL 10 mL 9.0 mL 0.8 mg/mL 10 mg/mL 0.8 mL 10 mL 9.2 mL 10 mg/mL 0.6 mg/mL 0.6 mL 10 mL 9.4 mL 0.4 mg/mL 10 mg/mL 9.6 mL 0.4 mL 10 mL 10 mg/mL 0.2 mg/mL 9.8 mL 0.2 mL 10 mL Calculation Examples: 1) V1 (10 mg/mL) V1 (0.8 mg/mL) (10 mL) 2)Vol. of water to add (V2 - V1) V1 = 0.8 mL LFocus 650 words Page 2 of 3 120% 12:22 PM PType here to search 12 1 10/17/2019
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