Propose a pathway for the following compound to enter gluconeogenesis / glycolysis. In your pathway, include enzymes (you can use E1, E2, etc.) as well as any necessary cofactors.

Prostaglandins are a class of eicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate issues. They are responsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from he 20-carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG2, the immediate precursor of many different prostaglandins. Arachidonic Rate of formation Rate of formation of PGG2 with 10 mg/ml acid (mM) of PGG2 (mM/min) ibuprofen (mM/min) 0.285 21.3 13.3 0.342 24.0 15.4 0.513 30.3 20.6 0.855 38.4 28.2 2.00 49.8 41.5 The kinetic data given in the table are for the reaction catalyzed by a mutant of prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. mM/min Vmax mM Km buprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the…

Glucose 6-phosphate is also a precursor for the synthesis of nucleotides that are used to build DNA and RNA. The first step of this pathway is catalyzed by an enzyme called glucose 6-phosphate dehydrogenase. Assume that reactions in this pathway with double arrows are reversible under cellular conditions, while those with single arrows are effectively irreversible. Which of the following enzymes, if present in your fructose-6-phosphate solution, would most substantially decrease the amount of 6-phosphogluconolactone you produce? Phosphoglucomutase G6P Dehydrogenase G6P Isomerase Hexokinase PFK-1

Step 4 of the pentose phosphate pathway converts ribulose-5-phosphate to ribose-5-phosphate. Which glycolytic reaction does this reaction resemble and what type of enzyme catalyzes it?

Indicate the general type of enzyme that mediates each glycolysis reaction depicted below. (e.g. Transferase, Oxidoreductase, Kinase, Hydrolase, Lyase, or Isomerase) iii. O H Glyceraldehyde-3-phosphate → 1,3-Bisphosphoglycerate H-C-OH + NAD CH₂O-P + P₁ H-O- -C-H Glucose →→ Glucose-6-phosphate H b 0 OH HO OH H-C- 0 -H HO- H ОН H-C-OH + NADH +H+ CH₂O P P ATP ADP 1 H (P-O- -C- H H HO OH 2-Phosphoglycerate → Phosphoenolpyruvate 0 OH H ОН -O~(P) + HOH