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Propionic and isobutyric acids are separated in a capillary GC column with retention times of 380 s and 395 s, respectively, and base widths of 17.0 s and 18.0 s. An unretained air peak occurs at 10.0 s. Calculate the separation factor and the resolution for the two peaks.

Propionic and isobutyric acids are separated in a capillary GC column with retention times of 380 s and 395 s, respectively, and base widths of 17.0 s and 18.0 s. An unretained air peak occurs at 10.0 s. Calculate the separation factor and the resolution for the two peaks.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Propionic and isobutyric acids are separated in a capillary GC column with retention times of 380 s and 395 s, respectively, and base widths of 17.0 s and 18.0 s. An uretained air peak occurs at 10.0 s. Calculate the separation factor and the resolution for the two peaks.
Transcribed Image Text:Propionic and isobutyric acids are separated in a capillary GC column with retention times of 380 s and 395 s, respectively, and base widths of 17.0 s and 18.0 s. An uretained air peak occurs at 10.0 s. Calculate the separation factor and the resolution for the two peaks.
Wavenumber em Spectrum (a) 4000 3000 2000 1500 1000 500 100- 50- Spectrum (b) 4000 3000 2000 1500 1000 500 100- 50- Spectrum (c) 4000 3000 2000 1500 1000 500 100- 50- 0- Transmittance (%) Transmitance (%) Transmittance (%)
Transcribed Image Text:Wavenumber em Spectrum (a) 4000 3000 2000 1500 1000 500 100- 50- Spectrum (b) 4000 3000 2000 1500 1000 500 100- 50- Spectrum (c) 4000 3000 2000 1500 1000 500 100- 50- 0- Transmittance (%) Transmitance (%) Transmittance (%)
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