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Procedure Sml Take ml Top up to 50ml. Add 50 ul of Mix overnight culture mixture with 45 ml LB to Add fresh LB ampicillin to the the mixture analyse both broth OD600 The mixture is then Withdraw 3ml mixture to Overnight culture (E-coli + LB borth) Measure every 15 mins. Stop until 75 mins. - incubated incubator at 37°c 200rpm -analyse (OD600)record as zero time-point. at shaked Mix overnight culture Sml Take 3 ml Add ampicillin to mixture 50 ul of the mixture to No ampicilin B (control) with 45 ml LB broth analyse the OD600 Measure every 15 mins. Stop until 75 mins. The mixture is then incubated incubator at 37°c 200rpm Withdraw 3ml mixture to shaked - analyse (OD600)record as zero time-point. at Graph of Absorbance vs Time(mins) 1.4 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1 (Ampicilin) Group 1(Control) 1. Absorbance(600nm)

Procedure Sml Take ml Top up to 50ml. Add 50 ul of Mix overnight culture mixture with 45 ml LB to Add fresh LB ampicillin to the the mixture analyse both broth OD600 The mixture is then Withdraw 3ml mixture to Overnight culture (E-coli + LB borth) Measure every 15 mins. Stop until 75 mins. - incubated incubator at 37°c 200rpm -analyse (OD600)record as zero time-point. at shaked Mix overnight culture Sml Take 3 ml Add ampicillin to mixture 50 ul of the mixture to No ampicilin B (control) with 45 ml LB broth analyse the OD600 Measure every 15 mins. Stop until 75 mins. The mixture is then incubated incubator at 37°c 200rpm Withdraw 3ml mixture to shaked - analyse (OD600)record as zero time-point. at Graph of Absorbance vs Time(mins) 1.4 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1 (Ampicilin) Group 1(Control) 1. Absorbance(600nm)

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Procedure Add 50 ul of Take mixture analyse ml Top up to 50ml. Add fresh Mix 5ml 3 LB ampicillin to mixture the overnight culture to the both with 45 ml LB broth OD600 is Withdraw 3ml mixture to The mixture incubated then Measure every 15 mins. Stop until 75 mins. Overnight culture -analyse (OD600)record as zero time-point. at shaked (E-coli + LB borth) incubator at 37°c 200rpm Mix overnight culture 5ml Take 3 ml Add 50 ul of mixture to- No ampicilin ampicillin to the B (control) with 45 ml LB broth analyse the mixture OD600 The mixture is then Withdraw ixture to Measure every 15 mins. Stop until 75 mins. shaked - analyse (OD600)record as zero time-point. incubated at incubator at 37°c 200rpm Graph of Absorbance vs Time(mins) 14 1.2 1 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1(Ampicilin) -Group 1(Control) Absorbance(600nm)
Transcribed Image Text:Procedure Add 50 ul of Take mixture analyse ml Top up to 50ml. Add fresh Mix 5ml 3 LB ampicillin to mixture the overnight culture to the both with 45 ml LB broth OD600 is Withdraw 3ml mixture to The mixture incubated then Measure every 15 mins. Stop until 75 mins. Overnight culture -analyse (OD600)record as zero time-point. at shaked (E-coli + LB borth) incubator at 37°c 200rpm Mix overnight culture 5ml Take 3 ml Add 50 ul of mixture to- No ampicilin ampicillin to the B (control) with 45 ml LB broth analyse the mixture OD600 The mixture is then Withdraw ixture to Measure every 15 mins. Stop until 75 mins. shaked - analyse (OD600)record as zero time-point. incubated at incubator at 37°c 200rpm Graph of Absorbance vs Time(mins) 14 1.2 1 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1(Ampicilin) -Group 1(Control) Absorbance(600nm)
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