Homework Help is Here – Start Your Trial Now!
Science
Biology
Procedure Sml Take ml Top up to 50ml. Add 50 ul of Mix overnight culture mixture with 45 ml LB to Add fresh LB ampicillin to the the mixture analyse both broth OD600 The mixture is then Withdraw 3ml mixture to Overnight culture (E-coli + LB borth) Measure every 15 mins. Stop until 75 mins. - incubated incubator at 37°c 200rpm -analyse (OD600)record as zero time-point. at shaked Mix overnight culture Sml Take 3 ml Add ampicillin to mixture 50 ul of the mixture to No ampicilin B (control) with 45 ml LB broth analyse the OD600 Measure every 15 mins. Stop until 75 mins. The mixture is then incubated incubator at 37°c 200rpm Withdraw 3ml mixture to shaked - analyse (OD600)record as zero time-point. at Graph of Absorbance vs Time(mins) 1.4 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1 (Ampicilin) Group 1(Control) 1. Absorbance(600nm)

Procedure Sml Take ml Top up to 50ml. Add 50 ul of Mix overnight culture mixture with 45 ml LB to Add fresh LB ampicillin to the the mixture analyse both broth OD600 The mixture is then Withdraw 3ml mixture to Overnight culture (E-coli + LB borth) Measure every 15 mins. Stop until 75 mins. - incubated incubator at 37°c 200rpm -analyse (OD600)record as zero time-point. at shaked Mix overnight culture Sml Take 3 ml Add ampicillin to mixture 50 ul of the mixture to No ampicilin B (control) with 45 ml LB broth analyse the OD600 Measure every 15 mins. Stop until 75 mins. The mixture is then incubated incubator at 37°c 200rpm Withdraw 3ml mixture to shaked - analyse (OD600)record as zero time-point. at Graph of Absorbance vs Time(mins) 1.4 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1 (Ampicilin) Group 1(Control) 1. Absorbance(600nm)

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question

Write a short dicussion based on the graph

Procedure Add 50 ul of Take mixture analyse ml Top up to 50ml. Add fresh Mix 5ml 3 LB ampicillin to mixture the overnight culture to the both with 45 ml LB broth OD600 is Withdraw 3ml mixture to The mixture incubated then Measure every 15 mins. Stop until 75 mins. Overnight culture -analyse (OD600)record as zero time-point. at shaked (E-coli + LB borth) incubator at 37°c 200rpm Mix overnight culture 5ml Take 3 ml Add 50 ul of mixture to- No ampicilin ampicillin to the B (control) with 45 ml LB broth analyse the mixture OD600 The mixture is then Withdraw ixture to Measure every 15 mins. Stop until 75 mins. shaked - analyse (OD600)record as zero time-point. incubated at incubator at 37°c 200rpm Graph of Absorbance vs Time(mins) 14 1.2 1 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1(Ampicilin) -Group 1(Control) Absorbance(600nm)
Transcribed Image Text:Procedure Add 50 ul of Take mixture analyse ml Top up to 50ml. Add fresh Mix 5ml 3 LB ampicillin to mixture the overnight culture to the both with 45 ml LB broth OD600 is Withdraw 3ml mixture to The mixture incubated then Measure every 15 mins. Stop until 75 mins. Overnight culture -analyse (OD600)record as zero time-point. at shaked (E-coli + LB borth) incubator at 37°c 200rpm Mix overnight culture 5ml Take 3 ml Add 50 ul of mixture to- No ampicilin ampicillin to the B (control) with 45 ml LB broth analyse the mixture OD600 The mixture is then Withdraw ixture to Measure every 15 mins. Stop until 75 mins. shaked - analyse (OD600)record as zero time-point. incubated at incubator at 37°c 200rpm Graph of Absorbance vs Time(mins) 14 1.2 1 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1(Ampicilin) -Group 1(Control) Absorbance(600nm)
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Microbial nutrition
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS