Prepare six different concentrations of Bovine Serum Albumin (BSA) standard from a 2 mg/mL stock. Based on the volumes given, what are the final concentrations of BSA for each standard (1 - 6)? Tube 2.0 mg/ml 0.15 BSA (µl) M Bradford Protein Molarity Dilution A595 NaCl (µl) (mg/tube) from stock Reagent |(ml) 1 100 1.0 2 20 80 1.0 3 40 60 1.0 4 60 40 1.0 5 80 20 1.0 6. 100 1.0 The molecular weight (Mw) for Bovine Serum Albumin (BSA) is 66,430.3 g/mol. Calculate the molarity of BSA in each of your standards based on this value and the mg/mL concentrations you derived above.

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Prepare six different concentrations of Bovine
Serum Albumin (BSA) standard from a 2
mg/mL stock. Based on the volumes given,
what are the final concentrations of BSA for
each standard (1 - 6)?
Tube
2.0 mg/ml 0.15
BSA (µl)
M Bradford
Protein
Molarity
Dilution
A595
NaCl (µl)
(mg/tube)
from stock
Reagent
|(ml)
1
100
1.0
2
20
80
1.0
3
40
60
1.0
4
60
40
1.0
5
80
20
1.0
6.
100
1.0
The molecular weight (Mw) for Bovine Serum
Albumin (BSA) is 66,430.3 g/mol. Calculate the
molarity of BSA in each of your standards
based on this value and the mg/mL
concentrations you derived above.
Transcribed Image Text:Prepare six different concentrations of Bovine Serum Albumin (BSA) standard from a 2 mg/mL stock. Based on the volumes given, what are the final concentrations of BSA for each standard (1 - 6)? Tube 2.0 mg/ml 0.15 BSA (µl) M Bradford Protein Molarity Dilution A595 NaCl (µl) (mg/tube) from stock Reagent |(ml) 1 100 1.0 2 20 80 1.0 3 40 60 1.0 4 60 40 1.0 5 80 20 1.0 6. 100 1.0 The molecular weight (Mw) for Bovine Serum Albumin (BSA) is 66,430.3 g/mol. Calculate the molarity of BSA in each of your standards based on this value and the mg/mL concentrations you derived above.
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