Prepare six different concentrations of Bovine Serum Albumin (BSA) standard from a 2 mg/mL stock. Based on the volumes given, what are the final concentrations of BSA for each standard (1 - 6)? Tube 2.0 mg/ml 0.15 BSA (µl) M Bradford Protein Molarity Dilution A595 NaCl (µl) (mg/tube) from stock Reagent |(ml) 1 100 1.0 2 20 80 1.0 3 40 60 1.0 4 60 40 1.0 5 80 20 1.0 6. 100 1.0 The molecular weight (Mw) for Bovine Serum Albumin (BSA) is 66,430.3 g/mol. Calculate the molarity of BSA in each of your standards based on this value and the mg/mL concentrations you derived above.

Give the complete solution Given = Req= Solution= Conclusion=

3a.

Please solve the normality of the sample in eq/L and the (DO) content of the sample in mg/L using the following equation.

Standardization of a sodium hydroxide (NaOH) solution against potassium hydrogen phthalate (KHP) yielded the following results: Mass of KHP, g Volume of NaOH, mL 0.7987 38.29 0.8365 38.96 0.8104 38.51 0.8039 38.29 MM KHP: 204.22 MM NaOH: 40.00 a. Calculate the mean molar concentration of sodium hydroxide. (First, compute the concentration of NAOH in each titration then compute for its mean/average value.) b. Calculate the standard deviation. Please provide solutions for your answers. If you have used your calculator for calculating for mean and standard deviation, you can just provide the formula with the values indicated.

How many mEq of NaCl (MW: 58.5) are represented in 50 ml normal saline (0.9% NaCl) solution? Molecular weight of NaCl is 58.5. O 15.4 155 O 308 7.7 O 465 If a NaCl solution contains 20 mEq of NaCl in each teaspoonful, how many milliliters will provide 35 mEq of sodium ion to the patient? O 0.57 ml 8.75 ml O 0.71 ml O 26.25 ml 140 ml

6) Provide a sample calculation for determining the mass of glucose, in milligrams, in the cuvette that contains the assay solution for the 0.02M Glucose Standard. The molecular weight of glucose is 180 g/mol, or 180 mg/mmol.

Calculate the percent mass per volume, % (m/v), of a dextrose solution containing 7.00 g of dextrose in 3.00 × 10² mL of solution. Note that mass is not technically the same as weight, but the abbreviation % (w/v) is often used interchangeably with % (m/v). mass/volume %: % (m/v)

3. What units are used for dissolved oxygen in this experiment? What do they mean? The units are ppm (parts per million); meaning, the number of milligrams of molecular oxygen in every million milliliters of solution. The units are mg/ml; meaning, the number of milligrams of molecular oxygen in every million milliliters of solution. The units are mg/L; meaning, the number of milligrams of molecular oxygen in every liter of solution. The units are mg/L; meaning, the number of milligrams of water in every liter of molecular oxygen.

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