Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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- how do i expand this into 1000 words The methodology employed to identify differentially expressed genes (DEGs) in breast cancer using RNA-Seq data involves several systematic steps integrating data retrieval, analysis, normalization, DEG identification, and functional annotation. Initially, raw RNA-Seq data is retrieved from the NCBI GEO database, specifically from dataset GSE216238 (Nakshatri, 2023), which encompasses samples from both breast cancer and normal tissue. Subsequently, the raw data was imported into Excel for initial analysis, leveraging its widespread availability and user-friendly interface. Gene expression data for breast cancer analysis was obtained from the Gene Expression Omnibus (GEO) database. The GEO homepage (https://www.ncbi.nlm.nih.gov/geo/) was accessed, and the "Query & Browse" tab was selected. Advanced Search: Under "Search GEO DataSets," an advanced search was conducted (https://www.ncbi.nlm.nih.gov/gds/advanced). Keywords "breast" and "cancer" were…arrow_forwardPLease help, double and triple check your answers, im using this to study, these questions are NOT graded they are PRACTICE problemsarrow_forwardQuestion 19 Match the following techniques with their purpose PCR Protein-protein interaction A. DNA amplification DNA sequencing CRISPR/Cas В. v Sanger dideoxy method C. Two hybrid method Protein localization D. GFP-tagging E. DNA editingarrow_forward
- for this question please help find pairwise differences and segregating sitesarrow_forwardPlease Write at your own words -Explain this How we can treatment for the genetic disorders by using gene therapy? Please explain at your own words, please.arrow_forwardPlease answer the question attached thank youarrow_forward
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