Physical characteristics of bacteria: carbohydrate metabolism. -Fermentation of Sugars -Hydrolysis of starch -Methyl red-voges-proskauer -Citrate utilization. Predicted results on each test for each organism: -S. Epidermidis -E.Coli -E. Aerogenes -P. Vulgarismo -B. Subtilis
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- missing a name or two from the list (Enterobacter aerogenes), and (Serratia rubidaea) - should be 20 Proteus Vulgaris (spelling) is eliminated under citrate so you can't have it at the end. Which test MR or VP - not both some of the above choices are strict aerobes and some are facultative anaerobes. This is helpful to first eliminate a set from your possibilities (look them up to figure this out)At first eliminate the organisms that *cannot* do something your organism *can*. This means you have a positive test result and you eliminate those that are always negative for that result. That way you do not have to worry that you accidentally killed the unknown before you inoculated it. You Do still need to be concerned that you interpreted the test results correctly and did not introduce a contaminant that has the ability you were testing while your true unknown did not.Hello, I want you pleaseeee to make a dichotomous key based on provided experiments to identify a an unknown bacterium mixture. It can be E. coli, B. Bulgaris, B subtilis, b aerogenes, m leuteus, s. Aureus, and s epidermisis. Make it based on these possible experiments: phenol red broth, mr/vp test, catalase test, oxidase test, nitrate reduction test, citrate utilization test, Malonate utilization test, decarboxylation test, starch hydrolysis test, dna hydrolysis test, lipid hydrolysis test, gelatin hydrolysis test, urea hydrolysis, SIM medium, triple sugar iron agar test, blood agar test. So far we have run a MAC agar and PEAA plate of the bacterium1). SIM Tubes and TSI slants turn black if the organism is able to reduce iron compounds. For which organism(s) below would you expect a black reaction? Circle all that apply. Escherichia coli. Proteus vulgaris. Salmonella typhimurium. Alcaligenes faecalis. Shigella flexneri. 2). If Citrate is a sugar and fermentation generates an acid, why does the BromThymol Blue indicator in the media turn blue and not yellow? 3). Why is Salmonellosis associated with eggs? Can you get Salmonellosis from other animal sources?
- Laboratory Exercises in Microbiology Results for Metabolic Tests McLaughlin and Petersen Lactose Bacteria Gram stain Morphology Arrangement Fermentation Gelatinase Amylase Urease Citrate permease Tryptophanase (Indole test) Catalase (Simmon's citrate) Bacillus subtilis Corynebacterium xerosis Enterobacter aerogenes Escherichia coli Micrococcus luteus Pseudomonas aeruginosa Proteus vulgaris Staphylococcus аureus Streptococcus faecalis Serratia тarcescens The tests in the table above will be available for your practical exam. Use your results from Lab 5 to fill out this table. Results for the bacteria that were not tested in Lab 5 can be found on the Microbiology Review site on Blackboard.The nitrites part of the dipstick test is mainly looking for which family of bacteria? Enterobacteriaceae O Actinomycetaceae Halobacteriaceae O StreptomycetaceaeCan someone help me make a dichotomous key for for the species listed below Bacillus megaterium, Citrobacter freundii, Clostridium sporogenes, Enterobracter aerogenes, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enterica (Typhimurium), Serratia marcescens, Shigella sonnei Using these tests: TSA plates, Gram stain, catalase test, citrate slants, gelantinase tubes, oxidase test, PR Glucose broths, PR lactose Broths, PR sucrose broths, SIM tubes,Urease slants (not all but most)
- Which of the following test will be useful for initial distinction between Which of the following test will be useful for initial distinction bet Salmonella and Escherichia? Lactose fermentation in MacConkey Motility H2S production in Indole medium Glucose fermentation in KIAYou are given a mixed culture of 3 bacteria with following characteristics- 1) Bacteria 1 - Methicillin- resistant Staphylococcus aureus (MRSA) (does not ferment any of these 4 sugars but forms endospores and is sensitive to penicillin) 2) Bacteria 2 - Escherichia coli (can ferment glucose, maltose, sucrose and lactose) and also produce carbon dioxide gas on fermenting these 3) Bacteria 3 - Enterobacter aerogenes. (can ferment only sucrose and cannot ferment glucose, maltose and lactose. Also on fermenting sucrose it produces hydrogen sulphide gas.) Also they all give similar looking white-colored colonies on an agar plate. Question: Generate separate pure cultures of these 3 bacteria from the mixed culture using the following tools that you learned in the lab. Use any (some or all) of these tools to generate pure cultures as well as confirm them. Any other technique that you use should be one you learned in lab. No need to give detailed info about how to perform the technique but it…McConkey agar plate is used to determine which carbohydrates a bacteria can ferment if the bacteria can produce gelatinase if the organism can produce catalase which antibiotics a bacteria is susceptible to the type of hemolysis a bacteria is capable of producing
- We’re going to make our own yogurt using mixed bacteria cultures which contains Lactobacillus casei, Bifidobacterium longum, Lactobacillus bulgaricus, Streptococcus thermophiles, Lactobacillus acidophilus How can you tell if you successfully made yogurt? What are the functions of all different species in the mixed yogurt cultures?Lactose fermentation Negatíve Salmonella Positive Escherichia coli Shigella Citrobacter Serratia Klebsiella Proteus Enterobacter Phenylalanine deaminase activity Indole production Negative Salmonella Positive Positive E. coli Proteus Shigella Serratia Negative Citrobacter Klebsiella H,S production Enterobacțer Negative Serratia Salmonella Shigella Positive Motility Negative Klebsiella Positive Citrobacter Citrate utilization Enterobacter H2S production Positive Negative Shigella Serratia Negative Enterobacter Positive CitrobacterYour organism is a Gram-negative organism (Enterobacter aerogenes) You must discuss all the IMViC tests and Hydrogen sulfide test in DETAIL. How can this be explained in lamen terms the step by step results of enterobacter aerogenes? DISCUSS all the results. Discuss in a logical and step by step fashion how you concluded that you had a certain microorganism.You need to explain which observation(s) enabled you to identify your unknown microorganism (Enterobacter aerogenes).To convince your reader you need phrases like; This observation “suggested” that my organism is Enterobacter aerogenes This observation “confirmed” that my organism is Enterobacter aerogenes