One of the main differences between TLC and column chromatography is the direction in which the mobile phase moves. Group of answer choices True False

In the diagram of TLC for column chromatography, What does light faded looking spots (less or less product, etc.)? Which fractions contain the product? concentrated 1 2 3 4 14 26 15 27 16 28 17 29 5 6 7 8 18 19 30 31 32 20 33 9 21 10 22 11 23 12 13 24 25 34 35 36 37 38

QUESTION 5 In a chromatographic separation using a polar stationary phase and a mobile phase composed of 60% hexane and 40% ethyl acetate, two compounds, benzene (peak A) and toluene (peak B), are eluted. The width of peak A at its base is 1.2 min, the width of peak B at its base is 1.3 min, and the retention time of peak A is 5.2 min and peak B is 3.5 min. What is the resolution (Rs) between these two peaks and is it acceptable? R = 1.36. It is not an acceptable resolution. R = 0.74. It is an acceptable resolution. R = 1.36. It is an acceptable resolution. R = 0.74. It is not an acceptable resolution.