One of the important reactions in the biochemical pathway glycolysis is the reaction of glucose-6-phosphate (G6P) to form fructose-6-phosphate (F6P): G6P F6P AG°=1.7 kJ Phosphoglucose Isom erase 6 CH,OPO 6CH2OPOS H 2- iCH,OH OH 1 но OH OH H 12 B OH OH он gluco se-6 -phosphate fructose-6-phosph ate Standard thermodynamic conditions imply the concentrations of G6P and F6P to be 1 M, however, in a typical cell, they are not even close to these values. Calcr kJ when the concentrations of G6P and F6P are 60.0 ×10M and 14.0 x10M respectively, assume the temperature is 37 °C. (R = 8.314 J/K-mol) O a. -0.1 kJ O b.-1.1 kJ O c. -2.1 kJ O d.-3.1 kJ O e. -4.1 kJ I-

Part A Which of the following is a product in the following reaction? glucose + fructose sucrose + H₂O sucrose fructose oxygen (O₂) glucose Submit My Answers Give Up Part B Examine the following reactions. Which of the following is true about compound C? Reaction 1 Reaction 2 Reaction 3 Reaction 4 G+H A+B → Submit C + D → E+F→ C is a product for reaction 2 only. C is the product of reaction 1 and a reactant for reaction 2. C is the reactant of reaction 1 and a product for reaction 2. C is a reactant for reaction 1 only. My Answers Give Up

N-NHPH CHO CH2OH H- C=N-NHPH Но- -H- 3 equiv Но- -H- 3 equiv Но- PHNHNH2 PHNHNH, H- H- OH H- -O- H- H- -HO- H- -HO- ČH2OH ČH2OH ČH2OH D-glucose osazone D-fructose + NH3 + PHNH2 + 2 H20 The final step in the formation of the osazone from glucose is the reaction of the keto imine with 2 equivalents of phenylhydrazine to yield the osazone plus ammonia. This reaction involves the following intermediate steps: 1. Addition of phenylhydrazine to the imine and proton transfer to yield intermediate 1; 2. Elimination of ammonia to yield phenylhydrazone 2; 3. Addition of phenylhydrazine to the ketone to yield tetrahedral intermediate 3; 4. Proton transfer yields carbinolamine 4; 5. Elimination of water yields the final product osazone. Write out the mechanism on a separate sh of paper and then draw the structure of tetrahedral intermediate 3. NH НО- -H H- -ОН H- -ОН ОН Glucose keto imine Previous Ne

If the following oligosaccharide was treated with an enzyme that cleaved only a 1,4 glycosidic bonds, what would be the products? OH CH₂OH OH CH₂OH OH CH₂OH OH OH OH OH CH₂OH CH₂OH a monosaccharide and a pentasaccharide a disaccharide and a tetrasaccharide two trisaccharides a monosaccharide, a disaccharide, and a trisaccharide O three disaccharides OH CH₂OH OH CH₂OH OH OH OH OH