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(B) 28 S 1 2 3 4 5 6 7 8 9 10 11 18 S 15% 28 S C 60% 18 S 15% 60% Sucrose S Questions 1. What molecules are detected by UV absorption at the top of the gradient (fraction 1)? 2. What complexes are detected by UV absorption in fraction 3? 3. What complexes are detected by UV absorption in fraction 5? 4. What complexes are detected by UV absorption in fractions 6-11? How does starvation affect the sedimentograms? How does it affect translation? Explain your conclusions. 6. Which step of translation (initiation or elongation) is mainly affected by starvation? OD₂ 260 1 2 3 (A) Control Starvation 4 5 6 7 8 9 10 11 Fraction

(B) 28 S 1 2 3 4 5 6 7 8 9 10 11 18 S 15% 28 S C 60% 18 S 15% 60% Sucrose S Questions 1. What molecules are detected by UV absorption at the top of the gradient (fraction 1)? 2. What complexes are detected by UV absorption in fraction 3? 3. What complexes are detected by UV absorption in fraction 5? 4. What complexes are detected by UV absorption in fractions 6-11? How does starvation affect the sedimentograms? How does it affect translation? Explain your conclusions. 6. Which step of translation (initiation or elongation) is mainly affected by starvation? OD₂ 260 1 2 3 (A) Control Starvation 4 5 6 7 8 9 10 11 Fraction

Essentials of Pharmacology for Health Professions
Essentials of Pharmacology for Health Professions
7th Edition
ISBN:9781305441620
Author:WOODROW
Publisher:WOODROW
Chapter5: Abbreviations And Systems Of Measurement
Section: Chapter Questions
Problem 2CRQ
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 Experiment The effect of starvation on protein synthesis in Caenorhabditis elegans was studied in this experiment. Worms were kept well fed (C, control) or starved (S) for 6 hours. Ribosomal fractions were isolated from the two groups and centrifuged in separate tubes through 15-60% sucrose density gradients. Fractions were collected from the top of the sucrose gradient. UV absorption at 260 nm (OD260) was measured (A), and RNA isolated from each fraction was analyzed by agarose gel electrophoresis (B).

(B) 28 S 1 2 3 4 5 6 7 8 9 10 11 18 S 15% 28 S C 60% 18 S 15% 60% Sucrose S Questions 1. What molecules are detected by UV absorption at the top of the gradient (fraction 1)? 2. What complexes are detected by UV absorption in fraction 3? 3. What complexes are detected by UV absorption in fraction 5? 4. What complexes are detected by UV absorption in fractions 6-11? How does starvation affect the sedimentograms? How does it affect translation? Explain your conclusions. 6. Which step of translation (initiation or elongation) is mainly affected by starvation?
Transcribed Image Text:(B) 28 S 1 2 3 4 5 6 7 8 9 10 11 18 S 15% 28 S C 60% 18 S 15% 60% Sucrose S Questions 1. What molecules are detected by UV absorption at the top of the gradient (fraction 1)? 2. What complexes are detected by UV absorption in fraction 3? 3. What complexes are detected by UV absorption in fraction 5? 4. What complexes are detected by UV absorption in fractions 6-11? How does starvation affect the sedimentograms? How does it affect translation? Explain your conclusions. 6. Which step of translation (initiation or elongation) is mainly affected by starvation?
OD₂ 260 1 2 3 (A) Control Starvation 4 5 6 7 8 9 10 11 Fraction
Transcribed Image Text:OD₂ 260 1 2 3 (A) Control Starvation 4 5 6 7 8 9 10 11 Fraction
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